ARG56964
anti-Liver FABP antibody [2G4]
anti-Liver FABP antibody [2G4] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [2G4] recognizes Liver FABP |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 2G4 |
| Isotype | IgG1, kappa |
| Target Name | Liver FABP |
| Antigen Species | Human |
| Immunogen | Recombinant fragment around aa. 1-127 of Human Liver FABP. |
| Conjugation | Un-conjugated |
| Alternate Names | FABPL; Fatty acid-binding protein, liver; Liver-type fatty acid-binding protein; L-FABP; Fatty acid-binding protein 1 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide and 10% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FABP1 |
| Gene Full Name | fatty acid binding protein 1, liver |
| Background | This gene encodes the fatty acid binding protein found in liver. Fatty acid binding proteins are a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. This protein and FABP6 (the ileal fatty acid binding protein) are also able to bind bile acids. It is thought that FABPs roles include fatty acid uptake, transport, and metabolism. [provided by RefSeq, Mar 2011] |
| Function | Binds free fatty acids and their coenzyme A derivatives, bilirubin, and some other small molecules in the cytoplasm. May be involved in intracellular lipid transport. [UniProt] |
| Calculated MW | 14 kDa |
Images (4) Click the Picture to Zoom In
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ARG56964 anti-Liver FABP antibody [2G4] ICC/IF image
Immunoflorescense: Hep3B cell line stained with ARG56964 anti-Liver FABP antibody [2G4] at 1:100 (Green).
DAPI (Blue) for nucleus staining.
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ARG56964 anti-Liver FABP antibody [2G4] FACS image
Flow Cytometry: Hep3B cell line stained with ARG56964 anti-Liver FABP antibody [2G4] at 2-5 µg for 1x10^6 cells. Secondary antibody: Goat anti-Mouse IgG Alexa fluor 488 conjugate.
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ARG56964 anti-Liver FABP antibody [2G4] IHC-P image
Immunohistochemistry: Paraffin embedded sections of Human colon cancer tissue stained with ARG56964 anti-Liver FABP antibody [2G4] at 1:100 for 2 hours at RT. Antigen Retrieval: Boil tissue section in 0.1M Sodium citrate buffer (pH 6.0) for 20 min.
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ARG56964 anti-Liver FABP antibody [2G4] WB image
Western blot: 1) 50 ng of FABP1 Recombinant protein, 2) 40 µg of HepG2 cell lysate, 3) 40 µg of Liver tissues lysate stained with ARG56964 anti-Liver FABP antibody [2G4] at 1:1000.
Specific References
Shigella flexneri Targets Human Colonic Goblet Cells by O Antigen Binding to Sialyl-Tn and Tn Antigens via Glycan-Glycan Interactions.
ICC/IF / Human
