ARG56700
anti-Leptin antibody
anti-Leptin antibody for ELISA,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Leptin |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ELISA, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Leptin |
| Antigen Species | Human |
| Immunogen | E.coli derived Recombinant Human Leptin. (MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSSKQKVTGL DFIPGLHPIL TLSKMDQTLA VYQQILTSMP SRNVIQISND LENLRDLLHV LAFSKSCHLP WASGLETLDS LGGVLEASGY STEVVALSRL QGSLQDMLWQ LDLSPGC) |
| Conjugation | Un-conjugated |
| Alternate Names | Leptin; Obese protein; OB; LEPD; Obesity factor; OBS |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | ~15 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.2) |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LEP |
| Gene Full Name | leptin |
| Background | This gene encodes a protein that is secreted by white adipocytes, and which plays a major role in the regulation of body weight. This protein, which acts through the leptin receptor, functions as part of a signaling pathway that can inhibit food intake and/or regulate energy expenditure to maintain constancy of the adipose mass. This protein also has several endocrine functions, and is involved in the regulation of immune and inflammatory responses, hematopoiesis, angiogenesis and wound healing. Mutations in this gene and/or its regulatory regions cause severe obesity, and morbid obesity with hypogonadism. This gene has also been linked to type 2 diabetes mellitus development. [provided by RefSeq, Jul 2008] |
| Function | May function as part of a signaling pathway that acts to regulate the size of the body fat depot. An increase in the level of LEP may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. [UniProt] |
| Calculated MW | 19 kDa |
Images (7) Click the Picture to Zoom In
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ARG56700 anti-Leptin antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56700 anti-Leptin antibody concentration is 0.5 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56700 anti-Leptin antibody WB image
Western blot: 250 - 0.24 ng of Human Leptin stained with ARG56700 anti-Leptin antibody, under reducing conditions.
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ARG56700 anti-Leptin antibody WB image
Western blot: 250 - 0.24 ng of Human Leptin stained with ARG56700 anti-Leptin antibody, under reducing conditions.
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ARG56700 anti-Leptin antibody IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded sections of Human breast invasive ductal carcinoma. The recommended ARG56700 anti-Leptin antibody concentration is 0.5 µg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detection system was used with a DAB chromogen. Antigen Retrieval: Boil tissue section in Sodium Citrate buffer (pH 6.0) followed by cooling at RT for 20 min.
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ARG56700 anti-Leptin antibody WB image
Western blot: 250 - 0.24 ng of Human Leptin stained with ARG56700 anti-Leptin antibody, under non-reducing conditions.
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ARG56700 anti-Leptin antibody WB image
Western blot: 250 - 0.24 ng of Human Leptin stained with ARG56700 anti-Leptin antibody, under non-reducing conditions.
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ARG56700 anti-Leptin antibody standard curve image
Sandwich ELISA: ARG56700 anti-Leptin antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56810 anti-Leptin antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.
