ARG65740
anti-Lamin B1 antibody
anti-Lamin B1 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Signaling Transduction antibody; NRF-2 Nuclear Localization Study antibody
Publication3
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Lamin B1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Lamin B1 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human Lamin B1 |
| Conjugation | Un-conjugated |
| Alternate Names | LMN2; ADLD; Lamin-B1; LMN; LMNB |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Jurkat |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | LMNB1 |
| Gene Full Name | lamin B1 |
| Background | The nuclear lamina consists of a two-dimensional matrix of proteins located next to the inner nuclear membrane. The lamin family of proteins make up the matrix and are highly conserved in evolution. During mitosis, the lamina matrix is reversibly disassembled as the lamin proteins are phosphorylated. Lamin proteins are thought to be involved in nuclear stability, chromatin structure and gene expression. Vertebrate lamins consist of two types, A and B. This gene encodes one of the two B type proteins, B1. Alternative splicing results in transcript variants and a duplication of this gene is associated with autosomal dominant adult-onset leukodystrophy (ADLD). [provided by RefSeq, Oct 2010] |
| Function | Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin. [UniProt] |
| Highlight | Related products: Lamin B1 antibodies; Lamin B1 Duos / Panels; Anti-Rabbit IgG secondary antibodies; |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Controls and Markers antibody; Signaling Transduction antibody; NRF-2 Nuclear Localization Study antibody |
| Calculated MW | 66 kDa |
| PTM | B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations. |
Images (4) Click the Picture to Zoom In
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ARG65740 anti-Lamin B1 antibody WB image
Western blot: 30 µg of Mouse spleen and Rat spleen lysates stained with ARG65740 anti-Lamin B1 antibody at 1:500 dilution.
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ARG65740 anti-Lamin B1 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG65740 anti-Lamin B1 antibody.
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ARG65740 anti-Lamin B1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon stained with ARG65740 anti-Lamin B1 antibody at 1:100 dilution.
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ARG65740 anti-Lamin B1 antibody WB image
Western blot: Jurkat cell lysate stained with ARG65740 anti-Lamin B1 antibody.
Customer's Feedback
Good
Review for anti-Lamin B1 antibody
Application：WB
Sample：Rat brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-Lamin B1 antibody
Application：WB
Sample：Mouse brain
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-Lamin B1 antibody
Application：WB
Sample：HeLa
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Pterostilbene Attenuates Particulate Matter-Induced Oxidative Stress, Inflammation and Aging in Keratinocytes
WB / Human
Effects of transcription factor EB on oxidative stress and apoptosis induced by high glucose in podocytes.
WB / Mouse
DAPK1-ERK signal mediates oxygen glucose deprivation reperfusion induced apoptosis in mouse N2a cells
WB / Mouse
