ARG42759

anti-LDHA / Lactate Dehydrogenase antibody

anti-LDHA / Lactate Dehydrogenase antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes LDHA / Lactate Dehydrogenase
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name LDHA / Lactate Dehydrogenase
Antigen Species Human
Immunogen Synthetic peptide derived from Human LDHA / Lactate Dehydrogenase.
Conjugation Un-conjugated
Alternate Names PIG19; Cell proliferation-inducing gene 19 protein; LDH muscle subunit; Renal carcinoma antigen NY-REN-59; HEL-S-133P; L-lactate dehydrogenase A chain; GSD11; LDHM; EC 1.1.1.27; LDH-M; LDH-A

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
ICC/IF1:50 - 1:100
IHC-P1:50 - 1:100
IP1:50
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa
Observed Size ~ 36 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16828 Mouse LDHA

GeneID: 3939 Human LDHA

Swiss-port # P00338 Human L-lactate dehydrogenase A chain

Swiss-port # P06151 Mouse L-lactate dehydrogenase A chain

Gene Symbol LDHA
Gene Full Name lactate dehydrogenase A
Background The protein encoded by this gene catalyzes the conversion of L-lactate and NAD to pyruvate and NADH in the final step of anaerobic glycolysis. The protein is found predominantly in muscle tissue and belongs to the lactate dehydrogenase family. Mutations in this gene have been linked to exertional myoglobinuria. Multiple transcript variants encoding different isoforms have been found for this gene. The human genome contains several non-transcribed pseudogenes of this gene. [provided by RefSeq, Sep 2008]
Cellular Localization Cytoplasm. [UniProt]
Calculated MW 37 kDa
PTM ISGylated. [UniProt]

Images (2) Click the Picture to Zoom In

  • ARG42759 anti-LDHA / Lactate Dehydrogenase antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human colon tissue stained with ARG42759 anti-LDHA / Lactate Dehydrogenase antibody.

  • ARG42759 anti-LDHA / Lactate Dehydrogenase antibody WB image

    Western blot: HeLa cell lysate stained with ARG42759 anti-LDHA / Lactate Dehydrogenase antibody.