ARG55622
anti-LC3 antibody
anti-LC3 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody
Publication3
Overview
| Product Description | Mouse Monoclonal antibody recognizes LC3 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 166AT1234 |
| Isotype | IgG1, kappa |
| Target Name | LC3 |
| Antigen Species | Human |
| Immunogen | Full length recombinant protein of Human LC3. |
| Conjugation | Un-conjugated |
| Alternate Names | MAP1A/MAP1B light chain 3 A; MAP1BLC3; LC3; MAP1 light chain 3-like protein 1; MAP1A/MAP1B LC3 A; Autophagy-related protein LC3 A; Microtubule-associated protein 1 light chain 3 alpha; MAP1ALC3; Microtubule-associated proteins 1A/1B light chain 3A; LC3A; ATG8E; Autophagy-related ubiquitin-like modifier LC3 A |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS and 0.09% (W/V) Sodium azide |
| Preservative | 0.09% (W/V) Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q91VR7 Mouse Microtubule-associated proteins 1A/1B light chain 3A Swiss-port # Q9H492 Human Microtubule-associated proteins 1A/1B light chain 3A |
|---|---|
| Gene Symbol | MAP1LC3A |
| Gene Full Name | microtubule-associated protein 1 light chain 3 alpha |
| Background | MAP1A and MAP1B are microtubule-associated proteins which mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. The protein encoded by this gene is one of the light chain subunits and can associate with either MAP1A or MAP1B. Two transcript variants encoding different isoforms have been found for this gene. The expression of variant 1 is suppressed in many tumor cell lines, suggesting that may be involved in carcinogenesis. [provided by RefSeq, Feb 2012] |
| Function | Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. [UniProt] |
| Cellular Localization | Cytoplasm, cytoskeleton. Endomembrane system; Lipid-anchor. Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Note=LC3-II binds to the autophagic membranes |
| Research Area | Cancer antibody; Cell Biology and Cellular Response antibody; Cell Death antibody; Metabolism antibody; Neuroscience antibody; Signaling Transduction antibody |
| Calculated MW | 14 kDa |
| PTM | The precursor molecule is cleaved by ATG4B to form the cytosolic form, LC3-I. This is activated by APG7L/ATG7, transferred to ATG3 and conjugated to phospholipid to form the membrane-bound form, LC3-II (PubMed:15187094). The Legionella effector RavZ is a deconjugating enzyme that produces an ATG8 product that would be resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. Phosphorylation at Ser-12 by PKA inhibits conjugation to phosphatidylethanolamine (PE). Interaction with MAPK15 reduces the inhibitory phosphorylation and increases autophagy activity. |
Images (3) Click the Picture to Zoom In
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ARG55622 anti-LC3 antibody ICC/IF image
Immunofluorescence: 4% PFA fixed (30 min) Mouse vascular smooth muscle cells stained with ARG55622 anti-LC3 antibody at 1:200 dilution, 25 ºC for 2 hours.
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ARG55622 anti-LC3 antibody IHC-P image
Immunohistochemistry: Muscle tissue of a diseased Mouse off Dox after 5 weeks on regular food, stained with ARG55622 anti-LC3 antibody. Several fibers that have autophagic vesicles throughout are visible.
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ARG55622 anti-LC3 antibody WB image
Western blot: 20 µg of MEF cell lysate stained with ARG55622 anti-LC3 antibody at 1:1000 dilution.
Customer's Feedback
Average
Review for anti-LC3 antibody
Application：WB
Sample：MEF
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-LC3 antibody
Application：IF/ICC
Sample：Mouse vascular smooth muscle cells
Fixation Buffer：4%PFA
Fixation Time：30 min
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：2 hrs
Primary Antibody Incubation Temperature：25 ºC
Specific References
Ginsenoside Rb1 alleviated concanavalin A-induced hepatocyte pyroptosis by activating mitophagy
WB, ICC/IF / Human, Mouse
β-Glucan produced by Lentinus edodes suppresses breast cancer progression via the inhibition of macrophage M2 polarization by integrating autophagy and inflammatory signals
WB / Human, Mouse
MicroRNA-145 Delay Plaque Erosion by Down-Regulating TGFβr Ii and Suppressing Autophagy of Vascular Smooth Muscle Cells.
WB, IHC-P, ICC/IF / Human
