ARG55335
anti-KPNA3 / IPOA4 antibody
anti-KPNA3 / IPOA4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Controls and Markers antibody; Signaling Transduction antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes KPNA3 / IPOA4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | KPNA3 / IPOA4 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human KPNA3 (NP_002258.2) |
| Conjugation | Un-conjugated |
| Alternate Names | Importin subunit alpha-4; SRP4; SRP1; Qip2; SRP1gamma; hSRP1; IPOA4; Karyopherin subunit alpha-3; SRP1-gamma; Importin alpha Q2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | A549 | ||||||||
| Observed Size | ~ 58 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KPNA3 |
| Gene Full Name | karyopherin alpha 3 (importin alpha 4) |
| Background | The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC), which consists of 60-100 proteins. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion while larger molecules are transported by an active process. The protein encoded by this gene belongs to the importin alpha family, and is involved in nuclear protein import. [provided by RefSeq, Jan 2009] |
| Function | Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Recognizes NLSs of influenza A virus nucleoprotein probably through ARM repeats 7-9. [UniProt] |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody |
| Calculated MW | 58 kDa |
Images (2) Click the Picture to Zoom In
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ARG55335 anti-KPNA3 / IPOA4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human esophagus stained with ARG55335 anti-KPNA3 / IPOA4 antibody at 1:100 dilution.
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ARG55335 anti-KPNA3 / IPOA4 antibody WB image
Western blot: 25 µg of A549 cell lysate stained with ARG55335 anti-KPNA3 / IPOA4 antibody at 1:1000 dilution.
