ARG55811
anti-KLF4 antibody
anti-KLF4 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Publication5
Overview
| Product Description | Rabbit Polyclonal antibody recognizes KLF4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | KLF4 |
| Antigen Species | Human |
| Immunogen | Recombinant protein of Human KLF4 |
| Conjugation | Un-conjugated |
| Alternate Names | GKLF; EZF; Epithelial zinc finger protein EZF; Krueppel-like factor 4; Gut-enriched krueppel-like factor |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | NIH/3T3 and BT474 | ||||||||
| Observed Size | ~ 52 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | KLF4 |
| Gene Full Name | Kruppel-like factor 4 (gut) |
| Background | This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015] |
| Function | Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription. [UniProt] |
| Calculated MW | 55 kDa |
Images (3) Click the Picture to Zoom In
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ARG55811 anti-KLF4 antibody ICC/IF image
Immunofluorescence: U2OS cells stained with ARG55811 anti-KLF4 antibody at 1:100 dilution.
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ARG55811 anti-KLF4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach cancer tissue stained with ARG55811 anti-KLF4 antibody at 1:200 dilution.
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ARG55811 anti-KLF4 antibody WB image
Western blot: NIH/3T3 and BT474 cell lysates stained with ARG55811 anti-KLF4 antibody.
Specific References
IL11 signaling mediates piR-2158 suppression of cell stemness and angiogenesis in breast cancer
WB / Mouse
MiR-7-5p/KLF4 signaling inhibits stemness and radioresistance in colorectal cancer
WB / Human
Dual Function of CCAT2 in Regulating Luminal Subtype of Breast Cancer Depending on the Subcellular Distribution
WB / Human
piRNA-823 Is Involved in Cancer Stem Cell Regulation Through Altering DNA Methylation in Association With Luminal Breast Cancer.
WB / Human
Long non-coding RNA CCAT2 promotes oncogenesis in triple-negative breast cancer by regulating stemness of cancer cells.
WB / Mouse
