ARG41836
anti-KDM1B / LSD2 antibody
anti-KDM1B / LSD2 antibody for Flow cytometry,ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat
Overview
Product Description | Rabbit Polyclonal antibody recognizes KDM1B / LSD2 |
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Tested Reactivity | Hu, Ms, Rat |
Tested Application | FACS, ICC/IF, IP, WB |
Host | Rabbit |
Clonality | Polyclonal |
Isotype | IgG |
Target Name | KDM1B / LSD2 |
Antigen Species | Human |
Immunogen | Synthetic peptide of Human KDM1B / LSD2. |
Conjugation | Un-conjugated |
Alternate Names | dJ298J15.2; Lysine-specific histone demethylase 1B; Flavin-containing amine oxidase domain-containing protein 1; C6orf193; EC 1.-.-.-; Lysine-specific histone demethylase 2; AOF1; LSD2; bA204B7.3 |
Application Instructions
Application Suggestion |
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Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||||
Positive Control | HeLa | ||||||||||
Observed Size | ~ 88 kDa |
Properties
Form | Liquid |
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Purification | Affinity purified. |
Buffer | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
Preservative | 0.02% Sodium azide |
Stabilizer | 50% Glycerol |
Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
Database Links |
Swiss-port # Q8CIG3 Mouse Lysine-specific histone demethylase 1B Swiss-port # Q8NB78 Human Lysine-specific histone demethylase 1B |
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Gene Symbol | KDM1B |
Gene Full Name | lysine (K)-specific demethylase 1B |
Background | Flavin-dependent histone demethylases, such as KDM1B, regulate histone lysine methylation, an epigenetic mark that regulates gene expression and chromatin function (Karytinos et al., 2009 [PubMed 19407342]).[supplied by OMIM, Oct 2009] |
Function | Histone demethylase that demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Required for de novo DNA methylation of a subset of imprinted genes during oogenesis. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and di-methylated 'Lys-4' of histone H3. Has no effect on tri-methylated 'Lys-4', mono-, di- or tri-methylated 'Lys-9', mono-, di- or tri-methylated 'Lys-27', mono-, di- or tri-methylated 'Lys-36' of histone H3, or on mono-, di- or tri-methylated 'Lys-20' of histone H4 (By similarity). [UniProt] |
Cellular Localization | Nucleus. [UniProt] |
Calculated MW | 92 kDa |
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