ARG57826
anti-JAK1 antibody
anti-JAK1 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Rat
Overview
| Product Description | Mouse Monoclonal antibody recognizes JAK1 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | ICC/IF, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Target Name | JAK1 |
| Antigen Species | Human |
| Immunogen | Purified recombinant fragment of Human JAK1. |
| Conjugation | Un-conjugated |
| Alternate Names | JTK3; Janus kinase 1; JAK-1; Tyrosine-protein kinase JAK1; JAK1A; JAK1B; EC 2.7.10.2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified by ammonium sulfate co-precipitation. |
| Buffer | PBS (pH 7.4), 0.03% Proclin300 and 50% Glycerol. |
| Preservative | 0.03% Proclin300 |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | JAK1 |
| Gene Full Name | Janus kinase 1 |
| Background | Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-alpha/beta and -gamma signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-alpha pathway, and between JAK1 and JAK2 in the interferon-gamma pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes. These kinases couple cytokine ligand binding to tyrosine phosphorylation of various known signaling proteins and of a unique family of transcription factors termed the signal transducers and activators of transcription, or STATs. [provided by RefSeq, Jul 2008] |
| Function | Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor. [UniProt] |
| Cellular Localization | Endomembrane system; Peripheral membrane protein. [UniProt] |
| Calculated MW | 133 kDa |
| PTM | Autophosphorylated (PubMed:7615558). Phosphorylated on tyrosine residues in response to interferon gamma signaling (PubMed:7615558). Dephosphorylation of Tyr-1034 and Tyr-1035 by PTPN2 negatively regulates cytokine-mediated signaling (PubMed:11909529). Ubiquitinated by RNF125; leading to its degradation by the proteasome. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG57826 anti-JAK1 antibody ICC/IF image
Immunofluorescence: HeLa cells fixed with 4% Paraformaldehyde and stained with ARG57826 anti-JAK1 antibody at 1:200 dilution.
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ARG57826 anti-JAK1 antibody WB image
Western blot: HeLa cell lysate stained with ARG57826 anti-JAK1 antibody at 1:1000 dilution.
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ARG57826 anti-JAK1 antibody IP image
Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG57826 anti-JAK1 antibody.
