ARG44506
anti-IRS4 antibody
anti-IRS4 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot,Flow cytometry and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes IRS4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Target Name | IRS4 |
| Antigen Species | Human |
| Immunogen | Human IRS4 recombinant protein |
| Conjugation | Un-conjugated |
| Alternate Names | IRS4; Insulin Receptor Substrate 4; IRS-4; PY160; 160 KDa Phosphotyrosine Protein; Phosphoprotein Of 160 Kda; Pp160; CHNG9; Py160 |
Application Instructions
| Application Suggestion |
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| Application Note | The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IRS4 |
| Gene Full Name | Insulin Receptor Substrate 4 |
| Background | IRS4 encodes the insulin receptor substrate 4, a cytoplasmic protein that contains many potential tyrosine and serine/threonine phosphorylation sites. Tyrosine-phosphorylated IRS4 protein has been shown to associate with cytoplasmic signalling molecules that contain SH2 domains. The IRS4 protein is phosphorylated by the insulin receptor tyrosine kinase upon receptor stimulation. |
| Function | Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin-stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May act as negative regulators of the IGF1 signaling pathway by suppressing the function of IRS1 and IRS2. |
| Cellular Localization | Cell membrane, Membrane |
| Calculated MW | 134 kDa |
| PTM | Phosphoprotein |
Images (9) Click the Picture to Zoom In
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ARG44506 anti-IRS4 antibody IHC-P image
Immunohistochemistry: Human intestine stained with ARG44506 anti-IRS4 antibody at 2 μg/mL dilution.
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ARG44506 anti-IRS4 antibody WB image
Western blot: 293T stained with ARG44506 anti-IRS4 antibody at 0.5 μg/mL dilution.
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ARG44506 anti-IRS4 antibody FACS image
Flow Cytometry: HeLa stained with ARG44506 anti-IRS4 antibody at 1 μg/10^6 cells dilution.
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ARG44506 anti-IRS4 antibody IHC-P image
Immunohistochemistry: Rat testis stained with ARG44506 anti-IRS4 antibody at 2 μg/mL dilution.
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ARG44506 anti-IRS4 antibody WB image
Western blot: Rat brain and Rat testis stained with ARG44506 anti-IRS4 antibody at 0.5 μg/mL dilution.
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ARG44506 anti-IRS4 antibody IHC-P image
Immunohistochemistry: Mouse testis stained with ARG44506 anti-IRS4 antibody at 2 μg/mL dilution.
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ARG44506 anti-IRS4 antibody WB image
Western blot: Mouse brain and Mouse testis stained with ARG44506 anti-IRS4 antibody at 0.5 μg/mL dilution.
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ARG44506 anti-IRS4 antibody IHC-P image
Immunohistochemistry: Human breast cancer stained with ARG44506 anti-IRS4 antibody at 2 μg/mL dilution.
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ARG44506 anti-IRS4 antibody IHC-P image
Immunohistochemistry: Human colon adenocarcinoma stained with ARG44506 anti-IRS4 antibody at 2 μg/mL dilution.
