ARG66285
anti-IL1 beta antibody [SQab1748]
anti-IL1 beta antibody [SQab1748] for ELISA,Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Pyroptosis Study antibody
Publication8
Overview
| Product Description | Mouse Monoclonal antibody [SQab1748] recognizes IL1 beta |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | ELISA, FACS, ICC/IF, IHC-P, WB |
| Specificity | Not cross-react with recombinant human IL-1 alpha in ELISA (5 µg/ml protein) and WB (100 ng protein). |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SQab1748 |
| Isotype | IgG2a |
| Target Name | IL1 beta |
| Antigen Species | Human |
| Immunogen | Recombinant Human IL1 beta protein. |
| Conjugation | Un-conjugated |
| Alternate Names | Interleukin-1 beta; IL1-BETA; IL-1; IL-1 beta; Catabolin; IL1F2 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | PBS (pH 7.4) and 0.01% Thimerosal. |
| Preservative | 0.01% Thimerosal |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | IL1B |
| Gene Full Name | interleukin 1, beta |
| Background | IL1 beta protein is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. [provided by RefSeq, Jul 2008] |
| Function | IL1 beta is a potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells (PubMed:10653850). [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30330 Pyroptosis Antibody Panel Related products: IL1 beta antibodies; IL1 beta ELISA Kits; IL1 beta Duos / Panels; IL1 beta recombinant proteins; Anti-Mouse IgG secondary antibodies; Related news: HMGB1 in inflammation Inflammatory Cytokines Exploring Antiviral Immune Response RIP1 activation and pathogenesis of NASH |
| Research Area | Pyroptosis Study antibody |
| Calculated MW | 31 kDa |
| PTM | Activation of the IL1B precursor involves a CASP1-catalyzed proteolytic cleavage. Processing and secretion are temporarily associated. [UniProt] |
Images (7) Click the Picture to Zoom In
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ARG66285 anti-IL1 beta antibody [SQab1748] WB image
Western blot: HUVECs stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1:1000 dilution.
From Yulin Yuan et al. Mol Med Rep. (2022), doi: 10.3892/mmr.2022.12730, Fig. 3.(A).
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ARG66285 anti-IL1 beta antibody [SQab1748] WB image
Western blot: 20 μg of A431 and HeLa cell lysates stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1:4000 dilution.
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ARG66285 anti-IL1 beta antibody [SQab1748] ICC/IF image
Immunofluorescence: MCF7 cells stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1:200 dilution.
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ARG66285 anti-IL1 beta antibody [SQab1748] FACS image
Flow Cytometry: HeLa cells stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1 µg/ml (red) and without antibody control (black).
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ARG66285 anti-IL1 beta antibody [SQab1748] ICC/IF image
Immunofluorescence: HeLa cells were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66285 anti-IL1 beta antibody [SQab1748] (green) at 1:200 dilution, and cell nuclei were stained with Hoechst 33342 (blue).
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ARG66285 anti-IL1 beta antibody [SQab1748] WB image
Western blot: 50 µg of Mouse liver lysate stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1:5000 dilution.
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ARG66285 anti-IL1 beta antibody [SQab1748] WB image
Western blot: 50 µg of Rat liver lysate stained with ARG66285 anti-IL1 beta antibody [SQab1748] at 1:5000 dilution.
Customer's Feedback
Excellent
Review for anti-IL1 beta antibody [SQab1748]
Application：WB
Sample：Mouse brain (MCAO/R surgery)
Sample Loading Amount：30 μg
Primary Antibody Dilution Factor：1:3000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Excellent
Review for anti-IL1 beta antibody [SQab1748]
Application：WB
Sample：A431 and HeLa
Sample Loading Amount：20 μg
Primary Antibody Dilution Factor：1:4000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
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Abscisic acid ameliorates oxidative stress, inflammation, and apoptosis in thioacetamide-induced hepatic fibrosis by regulating the NF-кB signaling pathway in mice.
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