ARG56667

anti-IL1 alpha antibody

anti-IL1 alpha antibody for ELISA,Functional study,IHC-Frozen sections,Neutralizing,Western blot and Mouse

publication_link Publication2

Overview

Product Description Rabbit Polyclonal antibody recognizes IL1 alpha
Tested Reactivity Ms
Tested Application ELISA, FuncSt, IHC-Fr, Neut, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name IL1 alpha
Antigen Species Mouse
Immunogen E.coli derived Recombinant Mouse IL-1α.
(SAPYTYQSDL RYKLMKLVRQ KFVMNDSLNQ TIYQDVDKHY LSTTWLNDLQ QEVKFDMYAY SSGGDDSKYP VTLKISDSQL FVSAQGEDQP VLLKELPETP KLITGSETDL IFFWKSINSK NYFTSAAYPE LFIATKEQSR VHLARGLPSM TDFQIS)
Conjugation Un-conjugated
Alternate Names IL-1 alpha; Interleukin-1 alpha; IL1; IL1F1; Hematopoietin-1; IL1-ALPHA; IL-1A

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56777 as a detection antibody
FuncStAssay-dependent
IHC-Fr1.0 µg/ml
Neut0.0 3 - 0.05 µg/ml (To yield [ND50] of the biological activity of mIL - 1α (0.05 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 16175 Mouse IL1A

Swiss-port # P01582 Mouse Interleukin-1 alpha

Gene Symbol Il1a
Gene Full Name interleukin 1 alpha
Background The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is a pleiotropic cytokine involved in various immune responses, inflammatory processes, and hematopoiesis. This cytokine is produced by monocytes and macrophages as a proprotein, which is proteolytically processed and released in response to cell injury, and thus induces apoptosis. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. It has been suggested that the polymorphism of these genes is associated with rheumatoid arthritis and Alzheimer's disease. [provided by RefSeq, Jul 2008]
Function Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. [UniProt]
Highlight Related products:
IL1 alpha antibodies; IL1 alpha ELISA Kits; IL1 alpha recombinant proteins; Anti-Rabbit IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
Calculated MW 31 kDa

Images (6) Click the Picture to Zoom In

  • ARG56667 anti-IL1 alpha antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Colchicine injected Mouse brain (including the hippocampal fissure) tissue stained with ARG56667 anti-IL1 alpha antibody at 1.0 µg/ml, overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™) reagent.

  • ARG56667 anti-IL1 alpha antibody WB image

    Western blot: 250 - 0.24 ng of Mouse IL-1α stained with ARG56667 anti-IL1 alpha antibody, under reducing conditions.

  • ARG56667 anti-IL1 alpha antibody IHC-Fr image

    Immunohistochemistry: Frozen section of Colchicine injected Mouse brain (including the hippocampal fissure) tissue stained with ARG56667 anti-IL1 alpha antibody at 1.0 µg/ml, overnight at 4˚C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA™) reagent.

  • ARG56667 anti-IL1 alpha antibody WB image

    Western blot: 250 - 0.24 ng of Mouse IL-1α stained with ARG56667 anti-IL1 alpha antibody, under non-reducing conditions.

  • ARG56667 anti-IL1 alpha antibody WB image

    Western blot: 250 - 0.24 ng of Mouse IL-1α stained with ARG56667 anti-IL1 alpha antibody, under non-reducing conditions.

  • ARG56667 anti-IL1 alpha antibody standard curve image

    Sandwich ELISA: ARG56667 anti-IL1 alpha antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56777 anti-IL1 alpha antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.

Specific References

Fecal microbiota transplantation inhibits colorectal cancer progression: Reversing intestinal microbial dysbiosis to enhance anti-cancer immune responses

WB / Mouse

Hao Yu et al.
Front Microbiol.,  (2023)

publication_link

 

hr_line

IL-1α antibody inhibits dose-dependent exacerbation of eosinophilic inflammation by crude house-dust-mite antigen in the conjunctiva of an atopic keratoconjunctivitis mouse model.

FuncSt / Mouse

Yukiko Shiraki et al.
Curr Eye Res.,  (2021)

publication_link

 

hr_line