ARG65273
anti-IGF2BP1 / IMP1 antibody
anti-IGF2BP1 / IMP1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes IGF2BP1 / IMP1. |
|---|---|
| Tested Reactivity | Hu |
| Predict Reactivity | Ms, Rat, Cow, Dog |
| Tested Application | IHC-P, WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_006537.3; NP_001153895.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IGF2BP1 / IMP1 |
| Antigen Species | Human |
| Immunogen | C-EKVFAEHKISYSGQ |
| Conjugation | Un-conjugated |
| Alternate Names | VICKZ1; CRDBP; IMP1; IMP-1; ZBP-1; ZBP1; CRD-BP; VICKZ family member 1; IGF2 mRNA-binding protein 1; IGF-II mRNA-binding protein 1; Zipcode-binding protein 1; Coding region determinant-binding protein; Insulin-like growth factor 2 mRNA-binding protein 1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q9NZI8 Human Insulin-like growth factor 2 mRNA-binding protein 1 |
|---|---|
| Background | This gene encodes a member of the insulin-like growth factor 2 mRNA-binding protein family. The protein encoded by this gene contains four K homology domains and two RNA recognition motifs. It functions by binding to the mRNAs of certain genes, including insulin-like growth factor 2, beta-actin and beta-transducin repeat-containing protein, and regulating their translation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 63 kDa |
| PTM | Phosphorylated. Phosphorylation may impair association with ACTB mRNA and hence abolishes translational repression (By similarity). |
Images (2) Click the Picture to Zoom In
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ARG65273 anti-IGF2BP1 / IMP1 antibody WB image
Western Blot: K562 lysate (35 µg protein in RIPA buffer) stained with ARG65273 anti-IGF2BP1 / IMP1 antibody at 0.3 µg/ml dilution.
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ARG65273 anti-IGF2BP1 / IMP1 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG65273 anti-IGF2BP1 / IMP1 antibody at 3.75 µg/ml dilution followed by AP-staining.
