ARG63880
anti-IFT88 / Polaris antibody
anti-IFT88 / Polaris antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes IFT88 / Polaris |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Dog, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Specificity | This antibody is expected to recognise both reported isoforms (NP_783195.2; NP_006522.2) |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | IFT88 / Polaris |
| Antigen Species | Human |
| Immunogen | C-KKRIDEDDFADEE |
| Conjugation | Un-conjugated |
| Alternate Names | Recessive polycystic kidney disease protein Tg737 homolog; hTg737; D13S1056E; Intraflagellar transport protein 88 homolog; TG737; TPR repeat protein 10; TTC10; Tetratricopeptide repeat protein 10; DAF19 |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | IHC-P: Antigen Retrieval: Microwaved tissue section in Tris/EDTA buffer (pH 9.0). WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13099 Human Intraflagellar transport protein 88 homolog Swiss-port # Q61371 Mouse Intraflagellar transport protein 88 homolog |
|---|---|
| Background | This gene encodes a member of the tetratrico peptide repeat (TPR) family. Mutations of a similar gene in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008] |
| Highlight | Related products: Anti-Goat IgG secondary antibodies; Related news: Gene therapy for retinitis pigmentosa (RP) |
| Research Area | Gene Regulation antibody |
| Calculated MW | 94 kDa |
Images (5) Click the Picture to Zoom In
-
ARG63880 anti-IFT88 / Polaris antibody ICC/IF image
Immunofluorescence: Paraformaldehyde fixed HepG2 cells permeabilized with 0.15% Triton. Cells were stained with ARG63880 anti-IFT88 / Polaris antibody (green) at 10 µg/ml dilution for 1 hour. DAPI (blue) for nuclear staining. Negative control: Unimmunized goat IgG (green) at 10 µg/ml dilution.
-
ARG63880 anti-IFT88 / Polaris antibody IHC-P image
Immunohistochemistry: paraffin embedded Human Kidney. (Microwaved antigen retrieval with Tris/EDTA buffer pH9) stained with ARG63880 anti-IFT88 / Polaris antibody at 3 µg/ml dilution followed by HRP-staining.
-
ARG63880 anti-IFT88 / Polaris antibody WB image
Western blot: 35 µg of Human kidney lysate (in RIPA buffer) stained with ARG63880 anti-IFT88 / Polaris antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
-
ARG63880 anti-IFT88 / Polaris antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HepG2 cells permeabilized with 0.5% Triton. Cells were stained with ARG63880 anti-IFT88 / Polaris antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
-
ARG63880 anti-IFT88 / Polaris antibody WB image
Western blot: 35 µg of HepG2 (A) and NIH/3T3 (B) cell lysates (in RIPA buffer) stained with ARG63880 anti-IFT88 / Polaris antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
