ARG56608

anti-IFN gamma antibody

anti-IFN gamma antibody for ELISA,Neutralizing,Western blot and Rat

Overview

Product Description Goat Polyclonal antibody recognizes IFN gamma
Tested Reactivity Rat
Tested Application ELISA, Neut, WB
Host Goat
Clonality Polyclonal
Isotype IgG
Target Name IFN gamma
Antigen Species Rat
Immunogen E.coli derived Recombinant Rat IFN-γ.
(MQGTLIESLE SLKNYFNSSS MDAMEGKSLL LDIWRNWQKD GNTKILESQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT NFFSNSKAKK DAFMSIAKFE VNNPQIQHKA VNELIRVIHQ LSPESSLRKR KRSRC)
Conjugation Un-conjugated
Alternate Names IFN-gamma; Interferon gamma; Immune interferon; IFG; IFI

Application Instructions

Application Suggestion
Tested Application Dilution
ELISASandwich: 0.5 - 2.0 µg/ml with ARG56723 as a detection antibody
Neut60.0 - 65.0 ng/ml (To yield [ND50] of the biological activity of Rat IFN - γ (0.3 ng/ml) )
WB0.1 - 0.2 µg/ml
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Affinity purification with immunogen.
Buffer PBS (pH 7.2)
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol Ifng
Gene Full Name interferon gamma
Background This gene encodes a soluble cytokine that is a member of the type II interferon class. The encoded protein is secreted by cells of the both the innate and adaptive immune systems. The active protein is a homodimer that binds to the interferon gamma receptor which triggers a cellular response to viral and microbial inflections. Mutations in this gene are associated with an increased susceptibility to viral, bacterial and parasitic infections and to several autoimmune diseases. [provided by RefSeq, Sep 2015]
Function Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. [UniProt]
Highlight Related products:
IFN gamma antibodies; IFN gamma ELISA Kits; IFN gamma Duos / Panels; IFN gamma recombinant proteins; Anti-Goat IgG secondary antibodies;
Related news:
HMGB1 in inflammation
Inflammatory Cytokines
Calculated MW 19 kDa
PTM Proteolytic processing produces C-terminal heterogeneity, with proteins ending alternatively at Gly-150, Met-157 or Gly-161.

Images (3) Click the Picture to Zoom In

  • ARG56608 anti-IFN gamma antibody WB image

    Western blot: 250 - 0.24 ng of Rat IFN-γ stained with ARG56608 anti-IFN gamma antibody, under reducing conditions.

  • ARG56608 anti-IFN gamma antibody WB image

    Western blot: 250 - 0.24 ng of Rat IFN-γ stained with ARG56608 anti-IFN gamma antibody, under non-reducing conditions.

  • ARG56608 anti-IFN gamma antibody standard curve image

    Sandwich ELISA: ARG56608 anti-IFN gamma antibody as a capture antibody at 0.5 - 2.0 µg/ml combined with ARG56723 anti-IFN-gamma antibody (Biotin) as a detection antibody. Results of a typical standard run with optical density.