anti-IDH1 antibody (R132H) [SQab22257]

anti-IDH1 antibody (R132H) [SQab22257] for IHC-Formalin-fixed paraffin-embedded sections and Human

Cancer antibody; Metabolism antibody; Signaling Transduction antibody


Product Description Recombinant Rabbit Monoclonal antibody [SQab22257] recognizes IDH1 (R132H)
Tested Reactivity Hu
Tested Application IHC-P
Host Rabbit
Clonality Monoclonal
Clone SQab22257
Isotype IgG
Target Name IDH1
Antigen Species Human
Immunogen Synthetic peptide around the region of Human IDH1 R132H.
Conjugation Un-conjugated
Protein Full Name Isocitrate dehydrogenase [NADP] cytoplasmic
Alternate Names IDPC; EC; Cytosolic NADP-isocitrate dehydrogenase; IDP; HEL-S-26; HEL-216; Isocitrate dehydrogenase [NADP] cytoplasmic; IDH; PICD; IDCD; NADP; Oxalosuccinate decarboxylase

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:100 - 1:200
Application Note IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer (pH 9.0). Incubate the samples at RT (18-25°C) for 30 min.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control Human glioma (IDH1 R132H) tissue


Form Liquid
Purification Purification with Protein A.
Buffer PBS, 0.01% Sodium azide, 40% Glycerol and 0.05%BSA.
Preservative 0.01% Sodium azide
Stabilizer 40% Glycerol and 0.05%BSA
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.


Database Links

GeneID: 3417 Human IDH1

Swiss-port # O75874 Human Isocitrate dehydrogenase [NADP] cytoplasmic

Gene Symbol IDH1
Gene Full Name isocitrate dehydrogenase 1 (NADP+), soluble
Background Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Sep 2013]
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Research Area Cancer antibody; Metabolism antibody; Signaling Transduction antibody
Calculated MW 47 kDa
PTM Acetylation at Lys-374 dramatically reduces catalytic activity.

Images (1) Click the Picture to Zoom In

  • ARG66929 anti-IDH1 antibody (R132H) [SQab22257] IHC-P image

    Immunohistochemistry: Formalin/PFA-fixed and paraffin-embedded sections of Human glioma (IDH1 R132H) tissue stained with ARG66929 anti-IDH1 antibody (R132H) [SQab22257]. Antigen Retrieval: Heat tissue section in Tris-EDTA buffer (pH 9.0).