ARG22207
anti-Hsp 47 antibody [1C4-1A6]
anti-Hsp 47 antibody [1C4-1A6] for ICC/IF,Western blot and Human
Overview
| Product Description | Mouse Monoclonal antibody [1C4-1A6] recognizes Hsp 47 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1C4-1A6 |
| Isotype | IgG1, kappa |
| Target Name | Hsp 47 |
| Antigen Species | Human |
| Immunogen | Full length Human HSP47 |
| Conjugation | Un-conjugated |
| Alternate Names | SERPINH1; Serpin Family H Member 1; HSP47; SERPINH2; CBP1; CBP2; Serine (Or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 1, (Collagen Binding Protein 1); Serpin Peptidase Inhibitor, Clade H (Heat Shock Protein 47), Member 1, (Collagen Binding Protein 1); Cell Proliferation-Inducing Gene 14 Protein; Rheumatoid Arthritis-Related Antigen RA-A47; Arsenic-Transactivated Protein 3; Collagen Binding Protein 1; 47 KDa Heat Shock Protein; Heat Shock Protein 47; Serpin H1; Colligin; AsTP3; Serine (Or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 2, (Collagen-Binding Protein 2); Serine (Or Cysteine) Proteinase Inhibitor, Clade H (Heat Shock Protein 47), Member 2; Rheumatoid Arthritis Antigen A-47; Collagen-Binding Protein; Colligin-1; Colligin-2; RA-A47; PIG14; PPROM; OI10; Gp46 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.09% Sodium azide and 50% Glycerol |
| Preservative | 0.09% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | SERPINH1 |
| Gene Full Name | Serpin Family H Member 1 |
| Background | This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum and plays a role in collagen biosynthesis as a collagen-specific molecular chaperone. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. Expression of this gene may be a marker for cancer, and nucleotide polymorphisms in this gene may be associated with preterm birth caused by preterm premature rupture of membranes. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, May 2011] |
| Function | Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen. [UniProt] |
| Cellular Localization | Endoplasmic reticulum. [UniProt] |
| Calculated MW | 46 kDa |
| PTM | Acetylationl; Glycoprotein Phosphoprotein. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG22207 anti-Hsp 47 antibody [1C4-1A6] ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22207 anti-Hsp 47 antibody [1C4-1A6] (green) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 100x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
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ARG22207 anti-Hsp 47 antibody [1C4-1A6] WB image
Western blot: 1) MW ladder, 20 µg of A431 lysates stained with ARG22207 anti-Hsp 47 antibody [1C4-1A6] (TBS for 60 min at RT) at 2) 1:250, 3) 1:500, or 4) 1:1000 dilutions. Block: 5% milk + TBST 1hr at RT.
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ARG22207 anti-Hsp 47 antibody [1C4-1A6] ICC/IF image
Immunocytochemistry: 2% Formaldehyde (20 min at RT) fixed Heat Shocked HeLa cells stained with ARG22207 anti-Hsp 47 antibody [1C4-1A6] (red) at 1:100 dilution (12 hours at 4°C). Counterstain: DAPI (blue) nuclear stain at 1:40000 for 120 min at RT. Magnification: 20x. Left: DAPI (blue) nuclear stain, Middle: Primary antibody, Right: Composite.
