ARG10169
anti-Helicobacter pylori CagA antibody [10E9]
anti-Helicobacter pylori CagA antibody [10E9] for ELISA,Western blot and Helicobacter pylori
Microbiology and Infectious Disease antibody
Overview
| Product Description | Mouse Monoclonal antibody [10E9] recognizes Helicobacter pylori CagA |
|---|---|
| Tested Reactivity | H. pylori |
| Tested Application | ELISA, WB |
| Specificity | Cross-reaction with other proteins has not been identified. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 10E9 |
| Isotype | IgG2a, kappa |
| Target Name | Helicobacter pylori CagA |
| Immunogen | A highly immunogenic 32kD CagA fragment, recombinant |
| Conjugation | Un-conjugated |
Application Instructions
| Application Note | ELISA: Clone 10E9 can be used as capture antibody in sandwich ELISA. Anti-CagA clone 10E9 coated wells detect recombinant CagA antigen in combination with HRP conjugated anti-CagA clone 5C6, clone 3C10 and clone 3C1. In addition, clone 10E9 selectively detected cell lysate of a CagA containing H. pylori strain when HRP conjugated anti-CagA clone 3C10 was used. Western Blot: This clone detects the 32 kD band corresponding to the molecular weight of the recombinant CagA antigen (Immunogen). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
|---|
Properties
| Form | Liquid |
|---|---|
| Purification | Protein G affinity purified |
| Buffer | 0.01M PBS (pH 7.0) |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Background | Helicobacter pylori (H. pylori), a spiral rod shaped gram-negative bacterium, is frequently found in the stomach. In infected populations, 10-20% may develop gastritis and gastric ulcer and 1-2% may develop cancer. The genome of H. pylori isolates from carriers with symptoms contains a 40kb pathogenicity island encoding H. pylori cytotoxin, cytotoxin associated gene A protein (CagA) and other virulence associated factors. CagA is used as a biomarker for virulent H. pylori strains. |
|---|---|
| Highlight | Related products: Helicobacter pylori antibodies; Helicobacter pylori ELISA Kits; Helicobacter pylori Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Tools for studying H. pylori diseases |
| Research Area | Microbiology and Infectious Disease antibody |
