ARG59687
anti-HMGN2 antibody
anti-HMGN2 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes HMGN2 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HMGN2 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to a sequence of Human HMGN2. (KKGKADAGKEGNNPAENGDAKTDQAQKAEGAGDAK) |
| Conjugation | Un-conjugated |
| Alternate Names | HMG17; High mobility group nucleosome-binding domain-containing protein 2; Non-histone chromosomal protein HMG-17 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | 18 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.9% NaCl, 0.2% Na2HPO4, 0.05% Sodium azide and 4% Trehalose. |
| Preservative | 0.05% Sodium azide |
| Stabilizer | 4% Trehalose |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P05204 Human Non-histone chromosomal protein HMG-17 |
|---|---|
| Gene Symbol | HMGN2 |
| Gene Full Name | high mobility group nucleosomal binding domain 2 |
| Background | The protein encoded by this gene binds nucleosomal DNA and is associated with transcriptionally active chromatin. Along with a similar protein, HMGN1, the encoded protein may help maintain an open chromatin configuration around transcribable genes. The protein has also been found to have antimicrobial activity against bacteria, viruses and fungi. [provided by RefSeq, Oct 2014] |
| Function | Binds to the inner side of the nucleosomal DNA thus altering the interaction between the DNA and the histone octamer. May be involved in the process which maintains transcribable genes in a unique chromatin conformation (By similarity). [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. Note=Cytoplasmic enrichment upon phosphorylation. [UniProt] |
| Calculated MW | 9 kDa |
| PTM | Phosphorylation favors cytoplasmic localization. [UniProt] |
Images (10) Click the Picture to Zoom In
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ARG59687 anti-HMGN2 antibody ICC/IF image
Immunofluorescence: MCF-7 cells were blocked with 10% goat serum and then stained with ARG59687 anti-HMGN2 antibody (red) at 2 µg/ml dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG59687 anti-HMGN2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59687 anti-HMGN2 antibody at 2 µg/ml, overnight at 4°C.
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ARG59687 anti-HMGN2 antibody WB image
Western blot: 50 µg of samples under reducing conditions. HeLa, A549 and U-87MG whole cell lysates stained with ARG59687 anti-HMGN2 antibody at 0.5 µg/ml, overnight at 4°C.
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ARG59687 anti-HMGN2 antibody FACS image
Flow Cytometry: Raji cells were blocked with 10% normal goat serum and then stained with ARG59687 anti-HMGN2 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG59687 anti-HMGN2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human oesophagus squama cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59687 anti-HMGN2 antibody at 2 µg/ml, overnight at 4°C.
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ARG59687 anti-HMGN2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human tonsil tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59687 anti-HMGN2 antibody at 2 µg/ml, overnight at 4°C.
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ARG59687 anti-HMGN2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human appendicitis tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59687 anti-HMGN2 antibody at 2 µg/ml, overnight at 4°C.
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ARG59687 anti-HMGN2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human gastric cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59687 anti-HMGN2 antibody at 2 µg/ml, overnight at 4°C.
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ARG59687 anti-HMGN2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovary cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59687 anti-HMGN2 antibody at 2 µg/ml, overnight at 4°C.
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ARG59687 anti-HMGN2 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human placenta tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG59687 anti-HMGN2 antibody at 2 µg/ml, overnight at 4°C.
