ARG66283
anti-HDGF antibody [SQab1746]
anti-HDGF antibody [SQab1746] for ELISA,Flow cytometry,ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat
Cancer; Growth factor
Overview
| Product Description | Mouse Monoclonal antibody [SQab1746] recognizes HDGF |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ELISA, FACS, ICC/IF, IHC, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SQab1746 |
| Isotype | IgG1 |
| Target Name | HDGF |
| Antigen Species | Human |
| Immunogen | Recombinant Human HDGF protein. |
| Conjugation | Un-conjugated |
| Alternate Names | HMG1L2; HMG-1L2; HDGF; High mobility group protein 1-like 2; Hepatoma-derived growth factor |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | PBS (pH 7.4) and 0.01% Thimerosal. |
| Preservative | 0.01% Thimerosal |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HDGF |
| Gene Full Name | hepatoma-derived growth factor |
| Background | HDGF is a member of the hepatoma-derived growth factor family. The encoded protein has mitogenic and DNA-binding activity and may play a role in cellular proliferation and differentiation. High levels of expression of this gene enhance the growth of many tumors. This gene was thought initially to be located on chromosome X; however, that location has been determined to correspond to a related pseudogene. Alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq, Jan 2016] |
| Function | HDGF Isoform 1: Acts as a transcriptional repressor (PubMed:17974029). Has mitogenic activity for fibroblasts (PubMed:11751870, PubMed:26845719). Heparin-binding protein (PubMed:15491618). Isoform 2: Does not have mitogenic activity for fibroblasts (PubMed:26845719). Does not bind heparin (PubMed:26845719). Isoform 3: Has mitogenic activity for fibroblasts (PubMed:26845719). Heparin-binding protein (PubMed:26845719). [UniProt] |
| Highlight | Related products: HDGF antibodies; HDGF ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: The most sensitive human HDGF ELISA kit The role of HDGF in tumor angiogenesis |
| Research Area | Cancer; Growth factor |
| Calculated MW | 27 kDa |
| PTM | Sumoylated with SUMO1. Sumoylation prevents binding to chromatin. [UniProt] |
Images (7) Click the Picture to Zoom In
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ARG66283 anti-HDGF antibody [SQab1746] ICC/IF image
Immunofluorescence: Cells (A549 and MCF-7) were fixed in 4% PFA, permeabilized with PBS containing 0.1% Triton X-100. Cells were stained with ARG66283 anti-HDGF antibody [SQab1746] (green) at 1:200 dilution, and cell nuclei were stained with Hoechst 33342 (blue).
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ARG66283 anti-HDGF antibody [SQab1746] FACS image
Flow Cytometry: 293T cells stained with ARG66283 anti-HDGF antibody [SQab1746] at 1 µg/ml (red) and without antibody control (black).
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ARG66283 anti-HDGF antibody [SQab1746] WB image
Western blot: 15 µg of 293T cell lysate stained with ARG66283 anti-HDGF antibody [SQab1746] at 1:5000 dilution.
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ARG66283 anti-HDGF antibody [SQab1746] ELISA image
ELISA: Titration curve of ARG66283 anti-HDGF antibody [SQab1746] in ELISA. Red: HDGF; Black: BSA (negative control).
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ARG66283 anti-HDGF antibody [SQab1746] WB image
Western blot: 20 µg of HuH7 cell lysate stained with ARG66283 anti-HDGF antibody [SQab1746] at 1:5000 dilution.
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ARG66283 anti-HDGF antibody [SQab1746] WB image
Western blot: 20 µg of 3T3 cell lysate stained with ARG66283 anti-HDGF antibody [SQab1746] at 1:5000 dilution.
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ARG66283 anti-HDGF antibody [SQab1746] WB image
Western blot: 50 µg Mouse liver and Rat liver lysates stained with ARG66283 anti-HDGF antibody [SQab1746] at 1:5000 dilution.
