ARG41844
anti-HDAC9 antibody
anti-HDAC9 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes HDAC9 |
|---|---|
| Tested Reactivity | Hu, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | HDAC9 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human HDAC9. |
| Conjugation | Un-conjugated |
| Alternate Names | HD7; MEF2-interacting transcription repressor MITR; Histone deacetylase-related protein; HDAC9B; Histone deacetylase 9; HDAC7; HDRP; HDAC9FL; MITR; HDAC7B; HD9; HD7b; EC 3.5.1.98; HDAC; Histone deacetylase 7B |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | K562 | ||||||||
| Observed Size | ~ 160 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | HDAC9 |
| Gene Full Name | histone deacetylase 9 |
| Background | Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene has sequence homology to members of the histone deacetylase family. This gene is orthologous to the Xenopus and mouse MITR genes. The MITR protein lacks the histone deacetylase catalytic domain. It represses MEF2 activity through recruitment of multicomponent corepressor complexes that include CtBP and HDACs. This encoded protein may play a role in hematopoiesis. Multiple alternatively spliced transcripts have been described for this gene but the full-length nature of some of them has not been determined. [provided by RefSeq, Jul 2008] |
| Function | Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription. Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter. [UniProt] |
| Cellular Localization | Nucleus. [UniProt] |
| Calculated MW | 111 kDa |
| PTM | Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import. Sumoylated. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG41844 anti-HDAC9 antibody ICC/IF image
Immunofluorescence: HeLa cells stained with ARG41844 anti-HDAC9 antibody (red, nuclear staining).
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ARG41844 anti-HDAC9 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human brain tissue stained with ARG41844 anti-HDAC9 antibody.
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ARG41844 anti-HDAC9 antibody WB image
Western blot: K562 cell lysate stained with ARG41844 anti-HDAC9 antibody.
