ARG62999

anti-HBV surface antigen / HBsAg antibody [HB3]

anti-HBV surface antigen / HBsAg antibody [HB3] for ELISA,ICC/IF and HBV

Cancer antibody; Microbiology and Infectious Disease antibody

Overview

Product Description

Mouse Monoclonal antibody [HB3] recognizes Hepatitis B surface antigen / HBs Ag

Tested Reactivity HBV
Tested Application ELISA, ICC/IF
Specificity The clone HB3 recognizes following Hepatitis B virus (HBV) subtypes: ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-. Hepatitis B surface antigen (HBsAg) is a marker of infectivity.
Specificity of the antibody HB3 was verified by ELISA on panel of virus subtypes identified on International Workshop on HBsAg Subtypes (Paris, April 1975). HB3 does not cross-block with the antibody HB5.
Host Mouse
Clonality Monoclonal
Clone HB3
Isotype IgG1
Target Name HBV surface antigen / HBsAg
Antigen Species HBV
Immunogen Purified HbsAg from human plasma.
Conjugation Un-conjugated
Alternate Names large S protein; pre-S1/pre-S2/S; L glycoprotein; L-HBsAG; LHB; large surface protein; major surface antigen

Application Instructions

Application Suggestion
Tested Application Dilution
ELISAAssay-dependent
ICC/IFAssay-dependent
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form Liquid
Purification Purified from ascites by precipitation methods and ion exchange chromatography.
Purity > 95% (by SDS-PAGE)
Buffer PBS (pH 7.4) and 15 mM Sodium azide
Preservative 15 mM Sodium azide
Concentration 1 mg/ml
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 944569 HBV S

Gene Symbol S
Gene Full Name L-HBsAG
Background Hepatitis B virus (HBV) is a hepadnavirus which has a circular genome composed of partially double-stranded DNA. The HBV surface protein antigens (HBsAg) are comprised of large (LHBs), middle (MHBs) and small (SHBs, also called major) protein. LHBs contains preS1, prS2, and small protein. MHBs does not include preS1 protein and SHBs dose not include preS1 and preS2 proteins. HbsAg and its antibodies have been developed as biomarkers to monitor infection stage. Expression of preS1 and preS2 in tissue or serum are also important to reveal the mechanism of HBV infection.
Function The large envelope protein exists in two topological conformations, one which is termed 'external' or Le-HBsAg and the other 'internal' or Li-HBsAg. In its external conformation the protein attaches the virus to cell receptors and thereby initiating infection. This interaction determines the species specificity and liver tropism. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis. The large envelope protein also assumes fusion between virion membrane and endosomal membrane (Probable). In its internal conformation the protein plays a role in virion morphogenesis and mediates the contact with the nucleocapsid like a matrix protein.
The middle envelope protein plays an important role in the budding of the virion. It is involved in the induction of budding in a nucleocapsid independent way. In this process the majority of envelope proteins bud to form subviral lipoprotein particles of 22 nm of diameter that do not contain a nucleocapsid. [UniProt]
Highlight Related products:
HBsAg antibodies;
Research Area Cancer antibody; Microbiology and Infectious Disease antibody