ARG22617
anti-Granzyme B antibody [GB11]
anti-Granzyme B antibody [GB11] for CyTOF®-candidate,ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation and Human,Chimpanzee,Monkey,Rhesus Monkey
Overview
| Product Description | Mouse Monoclonal antibody [GB11] recognizes Granzyme B This antibody recognizes the serine protease Granzyme B, which is important in the induction of apoptosis in target cells by cytolytic lymphocytes (CTLs). Granzyme B plays a key role in the induction of apoptosis by CTLs. After delivery to the target cell, Granzyme B activates the cascade of caspases that finally results in cell death. In normal peripheral blood approximately 20% of CD8+ve T cells have been found to express Granzyme B. |
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| Tested Reactivity | Hu, Chimp, Mk, R. Mk |
| Tested Application | CyTOF®-candidate, ELISA, FACS, ICC/IF, IHC-Fr, IP |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | GB11 |
| Isotype | IgG1 |
| Target Name | Granzyme B |
| Antigen Species | Human |
| Immunogen | Purified Human Granzyme B. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 3.4.21.79; CTLA-1; CSP-B; Granzyme B; CTLA1; CCPI; CGL-1; CGL1; Cytotoxic T-lymphocyte proteinase 2; T-cell serine protease 1-3E; HLP; CTSGL1; in-2; Cathepsin G-like 1; SECT; Granzyme-2; Human lymphocyte protein; C11; CSPB; Lymphocyte protease |
Application Instructions
| Application Suggestion |
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| Application Note | FACS: Use 10 µl of the suggested working dilution to label 10^6 cells or 100 µl whole blood. Membrane permeabilisation is required for this application. ELISA: This antibody has been reported to function as a capture reagent in sandwich ELISA assays for soluble Granzyme B in conjunction with a biotin conjugated antibody as detection reagent. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein A. |
| Buffer | Tris buffered saline and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | GZMB |
| Gene Full Name | granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) |
| Background | Granzyme B is a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016] |
| Function | Granzyme B is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. [UniProt] |
| Highlight | Related products: Granzyme B antibodies; Granzyme B ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: CyTOF-candidate Antibodies Examining CTL/NK-mediated cytotoxicity by ELISA Anti-SerpinB9 therapy, a new strategy for cancer therapy |
| Calculated MW | 28 kDa |
Images (1) Click the Picture to Zoom In
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ARG22617 anti-Granzyme B antibody [GB11] standard curve image
Sandwich ELISA: ARG22617 anti-Granzyme B antibody [GB11] as a capture antibody and biotinylated Mouse anti Human granzyme B as a detection antibody with recombinant Human granzyme B as antigen. Detection is by HRP conjugated streptavidin and substrate. It results of a typical standard run with optical density reading at 450 nm. Human plasma samples are diluted at i:2 (green) and 1:4 (blue).
