ARG11035
anti-Granzyme B antibody [B18.1]
anti-Granzyme B antibody [B18.1] for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human
Publication1
Overview
| Product Description | Mouse Monoclonal antibody [B18.1] recognizes Granzyme B |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | B18.1 |
| Isotype | IgG2a |
| Target Name | Granzyme B |
| Antigen Species | Human |
| Immunogen | Recombinant protein corresponding to aa. 7-227 of Human Granzyme B. |
| Conjugation | Un-conjugated |
| Alternate Names | EC 3.4.21.79; CTLA-1; CSP-B; Granzyme B; CTLA1; CCPI; CGL-1; CGL1; Cytotoxic T-lymphocyte proteinase 2; T-cell serine protease 1-3E; HLP; CTSGL1; in-2; Cathepsin G-like 1; SECT; Granzyme-2; Human lymphocyte protein; C11; CSPB; Lymphocyte protease |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Purity | > 95% (by SDS-PAGE) |
| Buffer | PBS and 0.02% Sodium azide. |
| Preservative | 0.02% Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GZMB |
| Gene Full Name | granzyme B (granzyme 2, cytotoxic T-lymphocyte-associated serine esterase 1) |
| Background | Granzyme B is a member of the granzyme subfamily of proteins, part of the peptidase S1 family of serine proteases. The encoded preproprotein is secreted by natural killer (NK) cells and cytotoxic T lymphocytes (CTLs) and proteolytically processed to generate the active protease, which induces target cell apoptosis. This protein also processes cytokines and degrades extracellular matrix proteins, and these roles are implicated in chronic inflammation and wound healing. Expression of this gene may be elevated in human patients with cardiac fibrosis. [provided by RefSeq, Sep 2016] |
| Function | Granzyme B is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. [UniProt] |
| Highlight | Related products: Granzyme B antibodies; Granzyme B ELISA Kits; Anti-Mouse IgG secondary antibodies; Related news: Examining CTL/NK-mediated cytotoxicity by ELISA Anti-SerpinB9 therapy, a new strategy for cancer therapy |
| Calculated MW | 28 kDa |
Images (2) Click the Picture to Zoom In
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ARG11035 anti-Granzyme B antibody [B18.1] WB image
Western blot: Cytokine-induced killer cell stained with ARG11035 anti-Granzyme B antibody [B18.1].
From Cheng-Che Wu et al. Biomed Pharmacother. (2023), doi: 10.1016/j.biopha.2023.114732, Fig. 3G.
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ARG11035 anti-Granzyme B antibody [B18.1] IHC-P image
Immunohistochemistry: Mouse triple-negative breast cancer tumor stained with ARG11035 anti-Granzyme B antibody [B18.1].
From Cheng-Che Wu et al. Biomed Pharmacother. (2023), doi: 10.1016/j.biopha.2023.114732, Fig. 6.
Specific References
Combination of FAK inhibitor and cytokine-induced killer cell therapy: An alternative therapeutic strategy for patients with triple-negative breast cancer
IHC-P, WB / Human, Mouse
