ARG22247
anti-GluR1 antibody [S355-1]
anti-GluR1 antibody [S355-1] for ICC/IF,Immunohistochemistry,Western blot and Human,Mouse,Rat
Neuroscience antibody; Postsynaptic Receptor antibody
Overview
| Product Description | Mouse Monoclonal antibody [S355-1] recognizes GluR1 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC, WB |
| Specificity | Detects ~100kDa. Does not cross-react with GluR2. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | S355-1 |
| Isotype | IgG1 |
| Target Name | GluR1 |
| Antigen Species | Rat |
| Immunogen | Fusion protein around aa. 1-389 (extracellular N-terminus) of Rat GluR1 |
| Conjugation | Un-conjugated |
| Alternate Names | GLUH1; GluA1; GluR-1; Glutamate receptor ionotropic, AMPA 1; GluR-K1; GLUR1; HBGR1; AMPA-selective glutamate receptor 1; GluR-A; GLURA; Glutamate receptor 1 |
Application Instructions
| Application Suggestion |
|
||||||||
|---|---|---|---|---|---|---|---|---|---|
| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purification with Protein G. |
| Buffer | PBS (pH 7.4), 0.1% Sodium azide and 50% Glycerol |
| Preservative | 0.1% Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Gria1 |
| Gene Full Name | glutamate receptor, ionotropic, AMPA 1 |
| Background | Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. This gene belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate (AMPA) receptors. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. [UniProt] |
| Cellular Localization | Cell Junction, Cell membrane, Endoplasmic Reticulum |
| Highlight | Related products: GluR1 antibodies; GluR1 Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Viral-like capsids, new trans-synaptic mRNA transport mechanism |
| Research Area | Neuroscience antibody; Postsynaptic Receptor antibody |
| Calculated MW | 102 kDa |
| PTM | Palmitoylated. Depalmitoylated upon glutamate stimulation. Cys-603 palmitoylation leads to Golgi retention and decreased cell surface expression. In contrast, Cys-829 palmitoylation does not affect cell surface expression but regulates stimulation-dependent endocytosis (By similarity). Phosphorylated at Ser-645. Phosphorylated at Ser-710 by PKC. Phosphorylated at Ser-849 by PKC, PKA and CAMK2. Phosphorylated at Ser-863 by PKC, PKA and PRKG2. |
Images (2) Click the Picture to Zoom In
-
ARG22247 anti-GluR1 antibody [S355-1] ICC/IF image
Immunofluorescence: Human Neuroblastoma cell line SK-N-BE. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: ARG22247 anti-GluR1 antibody [S355-1] at 1:100 for 60 min at RT. Secondary Antibody: Goat anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ARG22247 anti-GluR1 antibody [S355-1] (D) Composite.
-
ARG22247 anti-GluR1 antibody [S355-1] WB image
Western blot: 1) MW ladder, 2) 10 µg of Rat brain membrane lysates stained with ARG22247 anti-GluR1 antibody [S355-1] at 1:2000 dilution (60 min at RT). Block: 5% milk + TBST O/N at 4°C.
