ARG42603
anti-GSDME / DFNA5 antibody
anti-GSDME / DFNA5 antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GSDME / DFNA5 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GSDME / DFNA5 |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-260 of Human GSDME / DFNA5 (NP_004394.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Inversely correlated with estrogen receptor expression 1; Non-syndromic hearing impairment protein 5; ICERE-1 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | Mouse brain | ||||||||
| Observed Size | ~ 62 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O60443 Human Non-syndromic hearing impairment protein 5 Swiss-port # Q9Z2D3 Mouse Non-syndromic hearing impairment protein 5 homolog |
|---|---|
| Gene Symbol | DFNA5 |
| Gene Full Name | deafness, autosomal dominant 5 |
| Background | Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Function | Plays a role in the TP53-regulated cellular response to DNA damage probably by cooperating with TP53 (PubMed:16897187, PubMed:18223688). [Gasdermin-E, N-terminal]: Switches CASP3-mediated apoptosis induced by TNF or danger signals, such as chemotherapy drugs, to pyroptosis (PubMed:28459430, PubMed:27281216). Produced by the cleavage of GSDME by CASP3, perforates cell membrane and thereby induces pyroptosis. After cleavage, moves to the plasma membrane where it strongly binds to inner leaflet lipids, bisphosphorylated phosphatidylinositols, such as phosphatidylinositol (4,5)-bisphosphate (PubMed:28459430). Mediates secondary necrosis downstream of the mitochondrial apoptotic pathway and CASP3 activation as well as in response to viral agents (PubMed:28045099). Exhibits bactericidal activity (PubMed:27281216). [UniProt] |
| Cellular Localization | Gasdermin-E, N-terminal: Cell membrane. Gasdermin-E: Cytoplasm, cytosol. [UniProt] |
| Calculated MW | 55 kDa |
Images (3) Click the Picture to Zoom In
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ARG42603 anti-GSDME / DFNA5 antibody ICC/IF image
Immunofluorescence: PC-12 cells stained with ARG42603 anti-GSDME / DFNA5 antibody at 1:50 dilution.
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ARG42603 anti-GSDME / DFNA5 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung cancer tissue stained with ARG42603 anti-GSDME / DFNA5 antibody at 1:100 dilution.
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ARG42603 anti-GSDME / DFNA5 antibody WB image
Western blot: 25 µg of Mouse brain lysate stained with ARG42603 anti-GSDME / DFNA5 antibody at 1:1000 dilution.
Specific References
GSDME in Endothelial Cells: Inducing Vascular Inflammation and Atherosclerosis via Mitochondrial Damage and STING Pathway Activation
IHC-Fr / Mouse
