ARG41752

anti-GLUT1 antibody

anti-GLUT1 antibody for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes GLUT1
Tested Reactivity Hu, Ms, Rat
Tested Application FACS, ICC/IF, IHC-P, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name GLUT1
Antigen Species Human
Immunogen Synthetic peptide of Human GLUT1.
Conjugation Un-conjugated
Alternate Names DYT17; HepG2 glucose transporter; CSE; GLUT-1; GLUT; GLUT1DS; DYT18; HTLVR; PED; Glucose transporter type 1, erythrocyte/brain; DYT9; EIG12; GLUT1; Solute carrier family 2, facilitated glucose transporter member 1

Application Instructions

Application Suggestion
Tested Application Dilution
FACS1:50
ICC/IF1:50 - 1:200
IHC-P1:50 - 1:200
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HepG2
Observed Size ~ 47 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 20525 Mouse SLC2A1

GeneID: 24778 Rat SLC2A1

GeneID: 6513 Human SLC2A1

Gene Symbol SLC2A1
Gene Full Name solute carrier family 2 (facilitated glucose transporter), member 1
Background This gene encodes a major glucose transporter in the mammalian blood-brain barrier. The encoded protein is found primarily in the cell membrane and on the cell surface, where it can also function as a receptor for human T-cell leukemia virus (HTLV) I and II. Mutations in this gene have been found in a family with paroxysmal exertion-induced dyskinesia. [provided by RefSeq, Apr 2013]
Function Facilitative glucose transporter. This isoform may be responsible for constitutive or basal glucose uptake. Has a very broad substrate specificity; can transport a wide range of aldoses including both pentoses and hexoses. [UniProt]
Cellular Localization Cell membrane; Multi-pass membrane protein. Melanosome. Note=Localizes primarily at the cell surface. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. [UniProt]
Calculated MW 54 kDa

Images (3) Click the Picture to Zoom In

  • ARG41752 anti-GLUT1 antibody ICC/IF image

    Immunofluorescence: HepG2 cells stained with ARG41752 anti-GLUT1 antibody (green). Nuclear staining (blue).

  • ARG41752 anti-GLUT1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human cervix cancer tissue stained with ARG41752 anti-GLUT1 antibody.

  • ARG41752 anti-GLUT1 antibody WB image

    Western blot: HepG2 cell lysate stained with ARG41752 anti-GLUT1 antibody at 1:500 dilution.