ARG90005
anti-GLP1 Receptor antibody
anti-GLP1 Receptor antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Overview
| Product Description | Rabbit Polyclonal antibody recognizes GLP1 Receptor |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, IHC-P, WB |
| Specificity | Recognizes endogenous levels of GLP1 Receptor protein. |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GLP1 Receptor |
| Antigen Species | Human |
| Immunogen | KLH-conjugated synthetic peptide around the center region of Human GLP1 Receptor. |
| Conjugation | Un-conjugated |
| Alternate Names | GLP-1 receptor; GLP-1-R; Glucagon-like peptide 1 receptor; GLP-1R |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Sodium citrate buffer (pH 6.0) * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.42% Potassium phosphate (pH 7.3), 0.87% NaCl, 0.01% Sodium azide and 30% Glycerol |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 30% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GLP1R |
| Gene Full Name | glucagon-like peptide 1 receptor |
| Function | This is a receptor for glucagon-like peptide 1. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase. [UniProt] |
| Calculated MW | 53 kDa |
| PTM | N-glycosylation enhances cell surface expression and lengthens receptor half-life by preventing degradation in the ER. |
Images (3) Click the Picture to Zoom In
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ARG90005 anti-GLP1 Receptor antibody ICC/IF image
Immunocytochemistry: SKNSH cells stained with ARG90005 anti-GLP1 Receptor antibody (red). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at RT. Cells were stained with the primary antibody in 3% BSA-PBS and incubated overnight at 4°C in a hidified chamber. DAPI was used to stain the cell nuclei (blue).
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ARG90005 anti-GLP1 Receptor antibody IHC-P image
Immunohistochemistry: Formalin-fixed, paraffin embedded Human brain section stained with ARG90005 anti-GLP1 Receptor antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0), then incubated with the antibody at RT and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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ARG90005 anti-GLP1 Receptor antibody WB image
Western blot: SKNSH and HUVEC whole cell lysates stained with ARG90005 anti-GLP1 Receptor antibody.
