ARG52312
anti-GFAP antibody
anti-GFAP antibody for ICC/IF,Immunohistochemistry (PFA perfusion fixed frozen sections),IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Mouse,Rat
Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody
Publication3
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Glial Fibrillary Acidic Protein (GFAP) |
|---|---|
| Tested Reactivity | Ms, Rat |
| Tested Application | ICC/IF, IHC-FoFr , IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | GFAP |
| Antigen Species | Bovine |
| Immunogen | Recombinant and purified bovine GFAP |
| Conjugation | Un-conjugated |
| Alternate Names | Glial fibrillary acidic protein; ALXDRD; GFAP |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~50kDa GFAP protein. A lower band at ~45kDa is a proteolytic fragment derived from the GFAP molecule. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Neat Serum |
| Buffer | Neat serum |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | GFAP |
| Gene Full Name | glial fibrillary acidic protein |
| Background | GFAP is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008] |
| Function | GFAP is a class-III intermediate filament. It is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. [UniProt] |
| Highlight | Related Antibody Duos and Panels: ARG30006 NSC and Astrocyte Marker Antibody Duo (GFAP, Nestin) ARG30007 Astrocyte Marker Antibody Duo (Host: Goat, Rabbit) Related products: GFAP antibodies; GFAP Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Microglial help TAM-ing inflammation in the brain Astrocyte-to-neuron conversion for Parkinson's disease treatment |
| Research Area | Controls and Markers antibody; Developmental Biology antibody; Neuroscience antibody; Signaling Transduction antibody; Astrocyte Marker antibody; Astrocyte Maturation Marker antibody; Neuroinflammation antibody; Brain Injury IHC Study antibody |
| Calculated MW | 50 kDa |
| PTM | Phosphorylated by PKN1. |
Images (3) Click the Picture to Zoom In
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ARG52312 anti-GFAP antibody IHC-Fr image
Immunohistochemistry: Frozen section of Mouse C57BL/6Jnarl brain tissue. The tissue section was fixed by 4% formalin and blocked with BSA with 3% Goat serum, at RT for 1 hour. Tissue section was then stained with ARG52312 anti-GFAP antibody at 1:500 dilution, in PBS with 1% Goat serum, overnight at 4°C.
Blue: DAPI
Yellow: Venus reporter gene
Red: GFAP -
ARG52312 anti-GFAP antibody ICC/IF image
Immunofluorescence: Cultured neurons and glia stained with ARG52312 anti-GFAP antibody (red) and ARG52468 anti-Vimentin antibody (green) showing specific labeling of GFAP (red) and vimentin (green). Cells containing GFAP and vimentin appear yellow
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ARG52312 anti-GFAP antibody WB image
Western blot: Rat cortex lysate showing specific immunolabeling of the ~50 kDa GFAP protein stained with ARG52312 anti-GFAP antibody.
Customer's Feedback
Good
Review for anti-GFAP antibody
Application：IHC
Sample：Mouse C57BL/6Jnarl brain tissue
Sample Preparation Method：Frozen
Fixation Buffer：4% formalin
Primary Antibody Dilution Factor：1:500
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：Dylight 594
Good
Review for anti-GFAP antibody
Application：WB
Sample：Mouse brain and Rat brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:5000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
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