ARG41783

anti-Flotillin 1 antibody

anti-Flotillin 1 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat

Overview

Product Description Rabbit Polyclonal antibody recognizes Flotillin 1
Tested Reactivity Hu, Ms, Rat
Tested Application IHC-P, IP, WB
Host Rabbit
Clonality Polyclonal
Isotype IgG
Target Name Flotillin 1
Antigen Species Human
Immunogen Synthetic peptide of Human Flotillin 1.
Conjugation Un-conjugated
Alternate Names Flotillin-1

Application Instructions

Application Suggestion
Tested Application Dilution
IHC-P1:50 - 1:200
IP1:50
WB1:500 - 1:2000
Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.
Positive Control HeLa
Observed Size ~ 50 kDa

Properties

Form Liquid
Purification Affinity purified.
Buffer PBS (pH 7.4), 150 mM NaCl, 0.02% Sodium azide and 50% Glycerol.
Preservative 0.02% Sodium azide
Stabilizer 50% Glycerol
Storage Instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links

GeneID: 10211 Human FLOT1

GeneID: 14251 Mouse FLOT1

GeneID: 64665 Rat FLOT1

Gene Symbol FLOT1
Gene Full Name flotillin 1
Background Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq, Jul 2008]
Function May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. [UniProt]
Cellular Localization Cell membrane; Peripheral membrane protein. Endosome. Membrane, caveola; Peripheral membrane protein. Melanosome. Membrane raft. Note=Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Membrane-associated protein of caveola (By similarity). [UniProt]
Calculated MW 47 kDa

Images (2) Click the Picture to Zoom In

  • ARG41783 anti-Flotillin 1 antibody IHC-P image

    Immunohistochemistry: Paraffin-embedded Human uterus tissue stained with ARG41783 anti-Flotillin 1 antibody.

  • ARG41783 anti-Flotillin 1 antibody WB image

    Western blot: HeLa cell lysate stained with ARG41783 anti-Flotillin 1 antibody.