ARG58717
anti-Filamin B antibody
anti-Filamin B antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Filamin B |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Filamin B |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1686-1785 of Human Filamin B (NP_001157789.1). |
| Conjugation | Un-conjugated |
| Alternate Names | Actin-binding-like protein; ABP-280; TAP; ABP-280 homolog; LRS1; Truncated ABP; ABP-278; Fh1; Filamin-B; TABP; AOI; Thyroid autoantigen; FLN-B; FLN1L; Beta-filamin; SCT; Filamin-3; FH1; Filamin homolog 1; Truncated actin-binding protein |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | A431 | ||||||||
| Observed Size | 298 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FLNB |
| Gene Full Name | filamin B, beta |
| Background | This gene encodes a member of the filamin family. The encoded protein interacts with glycoprotein Ib alpha as part of the process to repair vascular injuries. The platelet glycoprotein Ib complex includes glycoprotein Ib alpha, and it binds the actin cytoskeleton. Mutations in this gene have been found in several conditions: atelosteogenesis type 1 and type 3; boomerang dysplasia; autosomal dominant Larsen syndrome; and spondylocarpotarsal synostosis syndrome. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Nov 2009] |
| Function | Connects cell membrane constituents to the actin cytoskeleton. May promote orthogonal branching of actin filaments and links actin filaments to membrane glycoproteins. Anchors various transmembrane proteins to the actin cytoskeleton. Interaction with FLNA may allow neuroblast migration from the ventricular zone into the cortical plate. Various interactions and localizations of isoforms affect myotube morphology and myogenesis. Isoform 6 accelerates muscle differentiation in vitro. [UniProt] |
| Cellular Localization | Cytoplasm, cell cortex, Cytoplasm, cytoskeleton, myofibril, sarcomere, Z line. [UniProt] |
| Calculated MW | 278 kDa |
| PTM | ISGylation prevents ability to interact with the upstream activators of the JNK cascade and inhibits IFNA-induced JNK signaling. Ubiquitination by a SCF-like complex containing ASB2 isoform 2 leads to proteasomal degradation which promotes muscle differentiation. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG58717 anti-Filamin B antibody ICC/IF image
Immunofluorescence: HUVEC cells stained with ARG58717 anti-Filamin B antibody at 1:100 dilution.
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ARG58717 anti-Filamin B antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human liver injury stained with ARG58717 anti-Filamin B antibody at 1:100 dilution.
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ARG58717 anti-Filamin B antibody WB image
Western blot: 25 µg of A431 cell lysate stained with ARG58717 anti-Filamin B antibody at 1:1000 dilution.
