ARG52283
anti-FOX3 / NeuN antibody [1B7]
anti-FOX3 / NeuN antibody [1B7] for ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat,Bovine,Cat,Chicken,Dog,Goat,Pig
Controls and Markers antibody; Gene Regulation antibody; Neuroscience antibody
Publication5
Overview
| Product Description | Mouse Monoclonal antibody [1B7] recognizes FOX3 / NeuN |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Cat, Chk, Dog, Goat, Pig |
| Tested Application | ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 1B7 |
| Isotype | IgG2a |
| Target Name | FOX3 / NeuN |
| Antigen Species | Human |
| Immunogen | Recombinant protein taken from the N-terminus of human FOX3 expressed in and purified from E. Coli |
| Conjugation | Un-conjugated |
| Alternate Names | RNA binding protein fox-1 homolog 3; NEUN; FOX-3; HRNBP3; Fox-1 homolog C; FOX3 |
Application Instructions
| Application Suggestion |
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Application Note | Specific for the ~46/48k FOX3 protein doublet * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity Purified |
| Buffer | PBS and 10 mM Sodium azide. |
| Preservative | 10 mM Sodium azide |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | RBFOX3 |
| Gene Full Name | RNA binding protein, fox-1 homolog (C. elegans) 3 |
| Background | FOX3, also known as NeuN and hexaribonucleotide binding protein 3, is a neuron-specific RNA binding nuclear protein involved in the regulation of pre-mRNA alternative splicing (Kim et al., 2009). FOX3 dependent alternative splicing of Numb has recently been shown to play an important role in the progression of neuronal differentiation during vertebrate development (Kim et al., 2013). |
| Highlight | Related products: FOX3 antibodies; Anti-Mouse IgG secondary antibodies; Related news: Stem cell and the regenerative medicine: Ready for the patients Neuronal Development Marker |
| Research Area | Controls and Markers antibody; Gene Regulation antibody; Neuroscience antibody |
| Calculated MW | 34 kDa |
Images (5) Click the Picture to Zoom In
-
ARG52283 anti-FOX3 / NeuN antibody [1B7] ICC/IF image
Immunofluorescence: Cultured Rat neurons stained with ARG52283 anti-FOX3 / NeuN antibody [1B7] showing strong nuclear and distal cytoplasmic staining of FOX3 in red and the complete absence of astrocyte staining, which are stained in green with ARG52313 anti-GFAP antibody.
-
ARG52283 anti-FOX3 / NeuN antibody [1B7] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Rat brain stem neurons stained with ARG52283 anti-FOX3 / NeuN antibody [1B7] (green) and counter stained with anti-MAP2 antibody (red). The nuclei of cells are revealed with DAPI in blue.
-
ARG52283 anti-FOX3 / NeuN antibody [1B7] WB image
Western Blot: Rat cortex lysate showing specific immunolabeling of the ~46/48k FOX3 protein stained with ARG52283 anti-FOX3 / NeuN antibody [1B7].
-
ARG52283 anti-FOX3 / NeuN antibody [1B7] WB image
Western blot: Rat brain, Rat brain (embryonic, E20) and Mouse brain lysates stained with ARG52283 anti-FOX3 / NeuN antibody [1B7] (green) at 1:1000 dilution.
-
ARG52283 anti-FOX3 / NeuN antibody [1B7] IHC-FoFr image
Immunohistochemistry: Adult Rat hippocampus stained with anti-c-Fos antibody (red) and DNA (blue). ARG52283 anti-FOX3 / NeuN antibody [1B7] (green) reveals strong nuclear and distal cytoplasmic staining for hippocampal neurons and the complete absence of staining of nonneuronal cells.
A few spontaneously active cells show in addition expression of c-Fos (arrowed). ARG52283 is therefore and excellent marker of neuronal activation.
Customer's Feedback
Good
Review for anti-FOX3 / NeuN antibody [1B7]
Application：WB
Sample：Mouse brain
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:2000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Impaired autophagic flux in the human brain after traumatic brain injury
IHC-P / Human
Paclitaxel induces cognitive impairment via necroptosis, decreased synaptic plasticity and M1 polarisation of microglia
ICC/IF / Mouse
Mesenchymal Stem Cell-Derived Exosomes Ameliorate Alzheimer's Disease Pathology and Improve Cognitive Deficits.
WB / Human
Ghrelin fiber projections from the hypothalamic arcuate nucleus into the dorsal vagal complex and the regulation of glycolipid metabolism.
IHC-Fr / Rat
Evolutional Characterization of Photochemically Induced Stroke in Rats: a Multimodality Imaging and Molecular Biological Study.
IHC-P / Rat
