ARG58667
anti-FECH antibody
anti-FECH antibody for IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Publication1
Overview
| Product Description | Rabbit Polyclonal antibody recognizes FECH |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | FECH |
| Antigen Species | Human |
| Immunogen | Recombinant fusion protein corresponding to aa. 1-200 of Human FECH (NP_000131.2). |
| Conjugation | Un-conjugated |
| Alternate Names | EPP; EC 4.99.1.1; Ferrochelatase, mitochondrial; FCE; Protoheme ferro-lyase; Heme synthase |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||
| Positive Control | Mouse brain | ||||||
| Observed Size | 50 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol. |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 50% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | FECH |
| Gene Full Name | ferrochelatase |
| Background | The protein encoded by this gene is localized to the mitochondrion, where it catalyzes the insertion of the ferrous form of iron into protoporphyrin IX in the heme synthesis pathway. Mutations in this gene are associated with erythropoietic protoporphyria. Two transcript variants encoding different isoforms have been found for this gene. A pseudogene of this gene is found on chromosome 3.[provided by RefSeq, May 2010] |
| Function | Catalyzes the ferrous insertion into protoporphyrin IX. [UniProt] |
| Cellular Localization | Mitochondrion inner membrane, Peripheral membrane protein, Matrix side. [UniProt] |
| Calculated MW | 48 kDa |
Images (2) Click the Picture to Zoom In
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ARG58667 anti-FECH antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human stomach stained with ARG58667 anti-FECH antibody at 1:100 dilution.
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ARG58667 anti-FECH antibody WB image
Western blot: 25 µg of Mouse brain lysate stained with ARG58667 anti-FECH antibody at 1:1000 dilution.
Specific References
The Effect of Photodynamic Therapy Using 5-Aminolevulinic Acid in Bone and Soft Tissue Sarcoma Cells
WB / Human,Mouse
