ARG23737
anti-F-actin antibody [NH3]
anti-F-actin antibody [NH3] for ELISA,Flow cytometry,ICC/IF,IHC-Frozen sections,Immunoprecipitation,Western blot and Human,Mouse,Rat,Rabbit
Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Cytochrome-C fractionation Study antibody; Inflammation Study antibody; Tag Internal Control antibody
Overview
| Product Description | Mouse Monoclonal antibody [NH3] recognizes F-actin. |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Rb |
| Tested Application | ELISA, FACS, ICC/IF, IHC-Fr, IP, WB |
| Specificity | The clone [NH3] recognises human Filamentous actin (F-actin) and reportedly also recognizes Globular actin (G-actin). The antibody binds to the N-terminal region of actin, but not to the extreme N-terminal 40 amino acids. In tissue sections the antibody stains the cytoplasm of macrophages strongly, and gives granular, localised nuclear staining of all cell types. As this is a mouse IgM antibody, an anti-mouse IgM secondary antibody is recommended to use to detect this primary antibody. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | NH3 |
| Isotype | IgM |
| Target Name | F-actin |
| Antigen Species | Human |
| Immunogen | Human monocytes and U937 cell line. |
| Conjugation | Un-conjugated |
| Alternate Names | PS1TP5BP1; BRWS1; Actin, cytoplasmic 1; Beta-actin |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified. |
| Buffer | PBS and 0.09% Sodium azide. |
| Preservative | 0.09% Sodium azide |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | ACTB |
| Background | This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008] |
| Function | Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. [UniProt] |
| Research Area | Controls and Markers antibody; Signaling Transduction antibody; Loading Control antibody; Cytochrome-C fractionation Study antibody; Inflammation Study antibody; Tag Internal Control antibody |
| Calculated MW | 42 kDa |
Images (1) Click the Picture to Zoom In
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ARG23737 anti-F-actin antibody [NH3] ICC/IF image
Immunofluorescence: Mouse cardiac microvascular endothelial cells were fixed with 4% PFA, permeabilized with 0.3% Triton X-100/PBS and then stained with ARG23737 anti-F-actin antibody [NH3] at 1:100 dilution.
