ARG55250
anti-EpCAM antibody
anti-EpCAM antibody for Immunoprecipitation,Western blot and Human
Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody
1
Overview
| Product Description | Mouse Monoclonal antibody recognizes EpCAM |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | IP, WB |
| Specificity | This antibody detects endogenous levels of EpCAM and does not cross-react with related proteins. |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Target Name | EpCAM |
| Antigen Species | Human |
| Immunogen | Purified recombinant Human EpCAM protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | MIC18; EGP; Tumor-associated calcium signal transducer 1; Epithelial glycoprotein 314; KSA; Ep-CAM; Epithelial cell surface antigen; Adenocarcinoma-associated antigen; HNPCC8; Cell surface glycoprotein Trop-1; EGP40; TACSTD1; KS1/4; hEGP314; Major gastrointestinal tumor-associated protein GA733-2; M4S1; MK-1; Epithelial glycoprotein; KS 1/4 antigen; ESA; DIAR5; EGP314; Epithelial cell adhesion molecule; EGP-2; TROP1; CD antigen CD326 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Purification | Affinity purification with immunogen. |
|---|---|
| Buffer | PBS (pH 7.4), 0.03% Proclin 300 and 50% Glycerol |
| Preservative | 0.03% Proclin 300 |
| Stabilizer | 50% Glycerol |
| Concentration | 6 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EPCAM |
| Gene Full Name | epithelial cell adhesion molecule |
| Background | EpCAM is a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy. [provided by RefSeq, Dec 2008] |
| Function | EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E. [UniProt] |
| Cellular Localization | Lateral cell membrane; Single-pass type I membrane protein. Cell junction > tight junction Note: Colocalizes with CLDN7 at the lateral cell membrane and tight junction. |
| Research Area | Controls and Markers antibody; Epithelial Marker antibody; Circulating Tumor Cells BioMarker antibody |
| Calculated MW | 35 kDa |
| PTM | Hyperglycosylated in carcinoma tissue as compared with autologous normal epithelia. Glycosylation at Asn-198 is crucial for protein stability. |
Images (2) Click the Picture to Zoom In
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ARG55250 anti-EpCAM antibody WB image
Western blot: 30 µg of HCT116 and HT29 cell lysates stained with ARG55250 anti-EpCAM antibody at 1:1000 dilution.
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ARG55250 anti-EpCAM antibody IP image
Immunoprecipitation: HCT116 cell lysates were immunoprecipitated and stained with ARG55250 anti-EpCAM antibody.
Customer's Feedback
Excellent
Review for anti-EpCAM antibody
Application：WB
Sample：HCT116 and HT29
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
