ARG11128
anti-EWSR1 / EWS antibody [5H7]
anti-EWSR1 / EWS antibody [5H7] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Dog,Horse
Overview
| Product Description | Mouse Monoclonal antibody [5H7] recognizes EWSR1 / EWS |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Dog, Hrs |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 5H7 |
| Isotype | IgG2b |
| Target Name | EWSR1 / EWS |
| Antigen Species | Human |
| Immunogen | Full-length Human EWSR1 / EWS. |
| Conjugation | Un-conjugated |
| Alternate Names | RNA-binding protein EWS; bK984G1.4; EWS-FLI1; Ewing sarcoma breakpoint region 1 protein; EWS; EWS oncogene |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Observed Size | ~ 80 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified |
| Buffer | PBS, 5 mM Sodium azide and 50% Glycerol. |
| Preservative | 5 mM Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EWSR1 |
| Gene Full Name | EWS RNA-binding protein 1 |
| Background | This gene encodes a multifunctional protein that is involved in various cellular processes, including gene expression, cell signaling, and RNA processing and transport. The protein includes an N-terminal transcriptional activation domain and a C-terminal RNA-binding domain. Chromosomal translocations between this gene and various genes encoding transcription factors result in the production of chimeric proteins that are involved in tumorigenesis. These chimeric proteins usually consist of the N-terminal transcriptional activation domain of this protein fused to the C-terminal DNA-binding domain of the transcription factor protein. Mutations in this gene, specifically a t(11;22)(q24;q12) translocation, are known to cause Ewing sarcoma as well as neuroectodermal and various other tumors. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 1 and 14. [provided by RefSeq, Jul 2009] |
| Function | Might normally function as a transcriptional repressor. EWS-fusion-proteins (EFPS) may play a role in the tumorigenic process. They may disturb gene expression by mimicking, or interfering with the normal function of CTD-POLII within the transcription initiation complex. They may also contribute to an aberrant activation of the fusion protein target genes. [UniProt] |
| Cellular Localization | Nucleus. Cytoplasm. Cell membrane. Note=Relocates from cytoplasm to ribosomes upon PTK2B/FAK2 activation. [UniProt] |
| Calculated MW | 68 kDa |
| PTM | Phosphorylated; calmodulin-binding inhibits phosphorylation of Ser-266. Highly methylated on arginine residues. Methylation is mediated by PRMT1 and, at lower level by PRMT8. [UniProt] |
Images (2) Click the Picture to Zoom In
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ARG11128 anti-EWSR1 / EWS antibody [5H7] ICC/IF image
Immunofluorescence: HeLa cells stained with ARG11128 anti-EWSR1 / EWS antibody [5H7] (green) and co-stained with anti-Vimentin antibody (red). Hoechst (blue) for nuclear staining.
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ARG11128 anti-EWSR1 / EWS antibody [5H7] WB image
Western blot: HeLa, HEK293, NIH/3T3, PC-12, Horse NBL6 and Dog A72 cell lysates stained with ARG11128 anti-EWSR1 / EWS antibody [5H7] at 1:1000 dilution.
