ARG43716
anti-ERK3 antibody
anti-ERK3 antibody for IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse
Overview
| Product Description | Rabbit Polyclonal antibody recognizes ERK3 |
|---|---|
| Tested Reactivity | Hu, Ms |
| Tested Application | IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | ERK3 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide of Human ERK3. |
| Conjugation | Un-conjugated |
| Protein Full Name | Mitogen-activated protein kinase 6 |
| Alternate Names | ERK3; HsT17250; p97-MAPK; EC 2.7.11.24; ERK-3; PRKM6; MAP kinase 6; MAP kinase isoform p97; p97MAPK; Mitogen-activated protein kinase 6; Extracellular signal-regulated kinase 3; MAPK 6 |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. | ||||||||
| Positive Control | NIH-3T3; HeLa | ||||||||
| Observed Size | 90-95 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 50 mM Tris-Glycine (pH 7.4), 150 mM NaCl, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Concentration | Batch dependent |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q16659 Human Mitogen-activated protein kinase 6 Swiss-port # Q61532 Mouse Mitogen-activated protein kinase 6 |
|---|---|
| Gene Symbol | MAPK6 |
| Gene Full Name | mitogen-activated protein kinase 6 |
| Background | The protein encoded by this gene is a member of the Ser/Thr protein kinase family, and is most closely related to mitogen-activated protein kinases (MAP kinases). MAP kinases also known as extracellular signal-regulated kinases (ERKs), are activated through protein phosphorylation cascades and act as integration points for multiple biochemical signals. This kinase is localized in the nucleus, and has been reported to be activated in fibroblasts upon treatment with serum or phorbol esters. [provided by RefSeq, Jul 2008] |
| Function | Atypical MAPK protein. Phosphorylates microtubule-associated protein 2 (MAP2) and MAPKAPK5. The precise role of the complex formed with MAPKAPK5 is still unclear, but the complex follows a complex set of phosphorylation events: upon interaction with atypical MAPKAPK5, ERK3/MAPK6 is phosphorylated at Ser-189 and then mediates phosphorylation and activation of MAPKAPK5, which in turn phosphorylates ERK3/MAPK6. May promote entry in the cell cycle (By similarity). [UniProt] |
| Cellular Localization | Cytoplasm. Nucleus. Translocates to the cytoplasm following interaction with MAPKAPK5. [UniProt] |
| Calculated MW | 83 kDa |
| PTM | Phosphorylated at Ser-189 by PAK1, PAK2 and PAK3 resulting in catalytic activation. Phosphorylated by MAPKAPK5 at other sites. Ubiquitination at Met-1 leads to degradation by the proteasome pathway. [UniProt] |
Images (3) Click the Picture to Zoom In
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ARG43716 anti-ERK3 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human colon cancer tissue stained with ARG43716 anti-ERK3 antibody at 1:20 dilution.
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ARG43716 anti-ERK3 antibody WB image
Western blot: HeLa cell lysates stained with ARG43716 anti-ERK3 antibody at 1:1000 dilution.
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ARG43716 anti-ERK3 antibody WB image
Western blot: NIH3T3 cell lysates stained with ARG43716 anti-ERK3 antibody at 1:1000 dilution.
