ARG10568

Name: anti-ERK1 + ERK2 antibody [B20-U]
Brand: arigo Biolaboratories
SKU: ARG10568
Price: 369 USD
Availability: InStock
Rating: 5.0 (3 reviews)

anti-ERK1 + ERK2 antibody [B20-U]

Datasheet

Publication1

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Price (USD)

50 μl

369.00

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Overview

Product Description	Rabbit Monoclonal antibody [B20-U] recognizes ERK1 + ERK2
Tested Reactivity	Hu, Ms, Rat
Tested Application	ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB
Host	Rabbit
Clonality	Monoclonal
Clone	B20-U
Isotype	IgG
Target Name	ERK1 + ERK2
Antigen Species	Human
Immunogen	Synthetic peptide around the C-terminus of Human ERK2.
Conjugation	Un-conjugated
Alternate Names	MAPK 2; MAPK 1; MAP kinase 2; ERK-2; p41; ERK; MAP kinase 1; PRKM2; PRKM1; EC 2.7.11.24; MAPK2; p40; Extracellular signal-regulated kinase 2; p38; Mitogen-activated protein kinase 2; Mitogen-activated protein kinase 1; ERK2; MAP kinase isoform p42; p42-MAPK; P42MAPK; p41mapk; ERT1

Application Instructions

Application Suggestion

Tested Application	Dilution
ELISA	Assay-dependent
ICC/IF	1:10
IHC-Fr	Assay-dependent
IHC-P	Assay-dependent
IP	Assay-dependent
WB	1:1000 - 1:5000

Application Note

* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Buffer	20 mM Tris-HCl (pH 8.0), 0.05% Sodium azide and 10 mg/ml BSA
Preservative	0.05% Sodium azide
Stabilizer	10 mg/ml BSA
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Database Links	GeneID: 116590 Rat MAPK1 GeneID: 26413 Mouse MAPK1 GeneID: 5594 Human MAPK1 Swiss-port # P28482 Human Mitogen-activated protein kinase 1 Swiss-port # P63085 Mouse Mitogen-activated protein kinase 1 Swiss-port # P63086 Rat Mitogen-activated protein kinase 1 More
Gene Symbol	MAPK1
Gene Full Name	mitogen-activated protein kinase 1
Background	ERK1 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008] ERK2 is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. One study also suggests that this protein acts as a transcriptional repressor independent of its kinase activity. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene. [provided by RefSeq, Jan 2014]
Function	Serine/threonine kinase which acts as an essential component of the MAP kinase signal transduction pathway. MAPK1/ERK2 and MAPK3/ERK1 are the 2 MAPKs which play an important role in the MAPK/ERK cascade. They participate also in a signaling cascade initiated by activated KIT and KITLG/SCF. Depending on the cellular context, the MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. The MAPK/ERK cascade plays also a role in initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors. About 160 substrates have already been discovered for ERKs. Many of these substrates are localized in the nucleus, and seem to participate in the regulation of transcription upon stimulation. However, other substrates are found in the cytosol as well as in other cellular organelles, and those are responsible for processes such as translation, mitosis and apoptosis. Moreover, the MAPK/ERK cascade is also involved in the regulation of the endosomal dynamics, including lysosome processing and endosome cycling through the perinuclear recycling compartment (PNRC); as well as in the fragmentation of the Golgi apparatus during mitosis. The substrates include transcription factors (such as ATF2, BCL6, ELK1, ERF, FOS, HSF4 or SPZ1), cytoskeletal elements (such as CANX, CTTN, GJA1, MAP2, MAPT, PXN, SORBS3 or STMN1), regulators of apoptosis (such as BAD, BTG2, CASP9, DAPK1, IER3, MCL1 or PPARG), regulators of translation (such as EIF4EBP1) and a variety of other signaling-related molecules (like ARHGEF2, FRS2 or GRB10). Protein kinases (such as RAF1, RPS6KA1/RSK1, RPS6KA3/RSK2, RPS6KA2/RSK3, RPS6KA6/RSK4, SYK, MKNK1/MNK1, MKNK2/MNK2, RPS6KA5/MSK1, RPS6KA4/MSK2, MAPKAPK3 or MAPKAPK5) and phosphatases (such as DUSP1, DUSP4, DUSP6 or DUSP16) are other substrates which enable the propagation the MAPK/ERK signal to additional cytosolic and nuclear targets, thereby extending the specificity of the cascade. [UniProt]
Calculated MW	41 kDa
PTM	Phosphorylated upon KIT and FLT3 signaling (By similarity). Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme. Undergoes regulatory phosphorylation on additional residues such as Ser-246 and Ser-248 in the kinase insert domain (KID) These phosphorylations, which are probably mediated by more than one kinase, are important for binding of MAPK1/ERK2 to importin-7 (IPO7) and its nuclear translocation. In addition, autophosphorylation of Thr-190 was shown to affect the subcellular localization of MAPK1/ERK2 as well. Ligand-activated ALK induces tyrosine phosphorylation. Dephosphorylated by PTPRJ at Tyr-187. Phosphorylation on Ser-29 by SGK1 results in its activation by enhancing its interaction with MAP2K1/MEK1 and MAP2K2/MEK2. DUSP3 and DUSP6 dephosphorylate specifically MAPK1/ERK2 and MAPK3/ERK1 whereas DUSP9 dephosphorylates a broader range of MAPKs. ISGylated.

Images (4) Click the Picture to Zoom In

ARG10568 anti-ERK1 + ERK2 antibody [B20-U] ICC/IF image

Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG10568 anti-ERK1 + ERK2 antibody [B20-U] at 1:10 dilution. Left: primary antibody (red). Right: DAPI (blue).

Secondary antibody: ARG21917 Goat anti-Rabbit IgG antibody (TRITC)
ARG10568 anti-ERK1 + ERK2 antibody [B20-U] WB image

Western blot: 20 µg of 293T and Jurkat cell lysates stained with ARG10568 anti-ERK1 + ERK2 antibody [B20-U] at 1:5000 dilution.
ARG10568 anti-ERK1 + ERK2 antibody [B20-U] IP image

Immunoprecipitation: Erk 1,2 immunoprecipitated from HEK 293 cells, visualized with ARG10568 anti-ERK1 + ERK2 antibody [B20-U] in WB analysis at 1:5000 dilution. 1) Marker, 2) 100 µg of HEK 293 stained with ARG10568 anti-ERK1 + ERK2 antibody [B20-U] (positive control), 3) Immunoprecipitation without primary antibody (negative control), and 4) Immunoprecipitated Erk 1,2 (~40 and ~45 kDa proteins) from HEK 293 cells (500 µg of crude protein extract) stained with ARG10568 anti-ERK1 + ERK2 antibody [B20-U].
ARG10568 anti-ERK1 + ERK2 antibody [B20-U] WB image

Western blot: 20 µg of Mouse brain and Rat brain lysates stained with ARG10568 anti-ERK1 + ERK2 antibody [B20-U] at 1:5000 dilution.