ARG66204
anti-EGFR antibody [SQab1726]
anti-EGFR antibody [SQab1726] for Flow cytometry,ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Immunoprecipitation,Western blot and Human,Mouse,Rat
Publication1
Overview
| Product Description | Recombinant Rabbit Monoclonal antibody [SQab1726] recognizes EGFR |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Tested Application | FACS, ICC/IF, IHC-P, IP, WB |
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone | SQab1726 |
| Isotype | IgG |
| Target Name | EGFR |
| Antigen Species | Human |
| Immunogen | Synthetic peptide around the C-terminus of EGFR. |
| Conjugation | Un-conjugated |
| Alternate Names | PIG61; ERBB1; Proto-oncogene c-ErbB-1; Receptor tyrosine-protein kinase erbB-1; NISBD2; Epidermal growth factor receptor; ERBB; HER1; EC 2.7.10.1; mENA |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS, 0.01% Sodium azide, 40% Glycerol and 0.05% BSA. |
| Preservative | 0.01% Sodium azide |
| Stabilizer | 40% Glycerol and 0.05% BSA |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | EGFR |
| Gene Full Name | epidermal growth factor receptor |
| Background | EGFR is a transmembrane glycoprotein. It is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. [provided by RefSeq, Jun 2016] |
| Function | EGFR: Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses (PubMed:2790960, PubMed:10805725, PubMed:27153536). Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF (PubMed:2790960, PubMed:7679104, PubMed:8144591, PubMed:9419975, PubMed:15611079, PubMed:12297049, PubMed:27153536, PubMed:20837704). Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules (PubMed:27153536). May also activate the NF-kappa-B signaling cascade (PubMed:11116146). Also directly phosphorylates other proteins like RGS16, activating its GTPase activity and probably coupling the EGF receptor signaling to the G protein-coupled receptor signaling (PubMed:11602604). Also phosphorylates MUC1 and increases its interaction with SRC and CTNNB1/beta-catenin (PubMed:11483589). Plays a role in enhancing learning and memory performance. Isoform 2 may act as an antagonist of EGF action. (Microbial infection) Acts as a receptor for hepatitis C virus (HCV) in hepatocytes and facilitates its cell entry. Mediates HCV entry by promoting the formation of the CD81-CLDN1 receptor complexes that are essential for HCV entry and by enhancing membrane fusion of cells expressing HCV envelope glycoproteins. [UniProt] |
| Highlight | Related products: EGFR antibodies; EGFR ELISA Kits; EGFR Duos / Panels; Related news: Cancer Pathology Markers (SQ clones) More than a biomarker, CA19-9 is a therapeutic target of pancreatic cancer |
| Calculated MW | 134 kDa |
| PTM | Phosphorylation at Ser-695 is partial and occurs only if Thr-693 is phosphorylated. Phosphorylation at Thr-678 and Thr-693 by PRKD1 inhibits EGF-induced MAPK8/JNK1 activation. Dephosphorylation by PTPRJ prevents endocytosis and stabilizes the receptor at the plasma membrane. Autophosphorylation at Tyr-1197 is stimulated by methylation at Arg-1199 and enhances interaction with PTPN6. Autophosphorylation at Tyr-1092 and/or Tyr-1110 recruits STAT3. Dephosphorylated by PTPN1 and PTPN2. Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occurs. Deubiquitination by OTUD7B prevents degradation. Ubiquitinated by RNF115 and RNF126 (By similarity). Methylated. Methylation at Arg-1199 by PRMT5 stimulates phosphorylation at Tyr-1197. |
Images (6) Click the Picture to Zoom In
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ARG66204 anti-EGFR antibody [SQab1726] WB image
Western blot: 30 µg of A549 and 293T cell lysates stained with ARG66204 anti-EGFR antibody [SQab1726] at 1:2000 dilution.
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ARG66204 anti-EGFR antibody [SQab1726] ICC/IF image
Immunofluorescence: A431 cells were fixed with 4% paraformaldehyde for 30 min at RT, permeabilized with 0.1% Triton X-100 for 10 min at RT then blocked with 10% goat serum for 30 min at RT. Cells were stained with ARG66204 anti-EGFR antibody [SQab1726] (green) at 1:200 and 4°C. DAPI (blue) was used as the nuclear counter stain.
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ARG66204 anti-EGFR antibody [SQab1726] FACS image
Flow Cytometry: A431 cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% TritonX-100 for 15 min. The cells were stained with ARG66204 anti-EGFR antibody [SQab1726] (red) at 1:200 dilution in 1x PBS/1% BSA for 30 min at RT, followed by Alexa Fluor® 488 labelled secondary antibody. Unlabelled sample (black) was used as a control.
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ARG66204 anti-EGFR antibody [SQab1726] IHC-P image
Immunohistochemistry: Formalin-fixed and paraffin-embedded Human lung cancer tissue stained with ARG66204 anti-EGFR antibody [SQab1726] at 1:200 dilution. Antigen Retrieval: Heat mediated was performed using Tris/EDTA buffer pH 9.0.
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ARG66204 anti-EGFR antibody [SQab1726] WB image
Western blot: 10 µg of PC-3, SH-SY5Y, A549, HeLa, A431, HaCat, Mouse skin and Rat skin lysates stained with ARG66204 anti-EGFR antibody [SQab1726] at 1:5000 dilution.
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ARG66204 anti-EGFR antibody [SQab1726] IP image
Immunoprecipitation: 0.4 mg of A431 whole cell lysate immunoprecipitated (1:10) and stained with ARG66204 anti-EGFR antibody [SQab1726]. 1) ARG66204 IP in A431 whole cell lysate, 2) PBS instead of ARG66204 in A431 whole cell lysate, and 3) A431 whole cell lysate, 10 µg (input).
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Review for anti-EGFR antibody [SQab1726]
Application：WB
Sample：A549 and 293T
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:2000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
16-Hydroxycleroda-3,13-dien-15,16-olide Induces Apoptosis in Human Bladder Cancer Cells through Cell Cycle Arrest, Mitochondria ROS Overproduction, and Inactivation of EGFR-Related Signalling Pathways.
WB / Human
