ARG63850
anti-Duffy / FY / DARC antibody
anti-Duffy / FY / DARC antibody for Flow cytometry,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human
Microbiology and Infectious Disease antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes Duffy / FY / DARC |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | FACS, IHC-P, WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_001116423.1; NP_002027.2). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Duffy / FY / DARC |
| Antigen Species | Human |
| Immunogen | HRAELSPSTENSSQLDFED-C |
| Conjugation | Un-conjugated |
| Alternate Names | Dfy; GpFy; GPD; FY; WBCQ1; Atypical chemokine receptor 1; CCBP1; CD antigen CD234; CD234; Plasmodium vivax receptor; DARC; Duffy antigen/chemokine receptor; Glycoprotein D; Fy glycoprotein |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Background | The protein encoded by this gene is a glycosylated membrane protein and a non-specific receptor for several chemokines. The encoded protein is the receptor for the human malarial parasites Plasmodium vivax and Plasmodium knowlesi. Polymorphisms in this gene are the basis of the Duffy blood group system. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Microbiology and Infectious Disease antibody |
| Calculated MW | 36 kDa |
Images (4) Click the Picture to Zoom In
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ARG63850 anti-Duffy / FY / DARC antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human lung tissue. Antigen Retrieval: Microwaved tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63850 anti-Duffy / FY / DARC antibody at 5 µg/ml dilution followed by AP-staining.
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ARG63850 anti-Duffy / FY / DARC antibody IHC-P image
Immunohistochemistry: paraffin embedded human kidney. (Microwaved antigen retrieval with citrate buffer pH 6) stained with ARG63850 anti-Duffy / FY / DARC antibody at 3 µg/ml dilution followed by HRP-staining.
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ARG63850 anti-Duffy / FY / DARC antibody WB image
Western blot: 35 µg of Human liver lysate (in RIPA buffer) stained with ARG63850 anti-Duffy / FY / DARC antibody at 0.3 µg/ml dilution and incubated at RT for 1 hour.
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ARG63850 anti-Duffy / FY / DARC antibody FACS image
Flow Cytometry: Paraformaldehyde-fixed HEK293 cells permeabilized with 0.5% Triton. Cells were stained with ARG63850 anti-Duffy / FY / DARC antibody (blue line) at 10 µg/ml dilution for 1 hour, followed by incubation with Alexa FluorR 488 labelled secondary antibody. IgG control: Unimmunized goat IgG (black line).
