ARG52266
anti-Doublecortin antibody [3 e1]
anti-Doublecortin antibody [3 e1] for ICC/IF,IHC-Frozen sections,Western blot and Human,Mouse,Rat,Bovine
Controls and Markers antibody; Neuroscience antibody; Hippocampal Neurogenesis Marker antibody; Immature Neuronal Cells Marker antibody
Overview
| Product Description | Mouse Monoclonal antibody [3 e1] recognizes Doublecortin |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov |
| Tested Application | ICC/IF, IHC-Fr, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 3 e1 |
| Isotype | IgG2A |
| Target Name | Doublecortin |
| Antigen Species | Human |
| Immunogen | Full length recombinant human protein expressed in and purified from E. coli |
| Conjugation | Un-conjugated |
| Alternate Names | LISX; Doublin; SCLH; Lissencephalin-X; Neuronal migration protein doublecortin; DC; Lis-X; DBCN; XLIS |
Application Instructions
| Application Suggestion |
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| Application Note | Specific for the ~35 & 45 k doublecortin protein. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | PBS, 5 mM Sodium azide and 50% Glycerol. |
| Preservative | 5 mM Sodium azide |
| Stabilizer | 50% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # O43602 Human Neuronal migration protein doublecortin Swiss-port # O88809 Mouse Neuronal migration protein doublecortin |
|---|---|
| Gene Symbol | DCX |
| Gene Full Name | doublecortin |
| Background | Doublecortin, or DCX, is a microtubule associated protein that is expressed almost exclusively in very early neuronal development (Brown et al., 2003), making it an excellent marker for developing neuronal cells. Defects in the DCX gene lead to X-linked lissencephaly which is characterized by a lack of normal folds on the surface of the brain resulting in a smooth cerebral cortex caused by abnormal migration of neurons during development (des Portes et al., 1998; Gleeson et al., 1998). |
| Highlight | Related Antibody Duos and Panels: ARG30093 Hippocampal Neurogenesis Marker Antibody Duo (Prox1, Dcx) Related products: Doublecortin antibodies; Doublecortin Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: "Pro-aging factor" tied to immune-related molecule Neuronal Development Marker |
| Research Area | Controls and Markers antibody; Neuroscience antibody; Hippocampal Neurogenesis Marker antibody; Immature Neuronal Cells Marker antibody |
| Calculated MW | 41 kDa |
| PTM | Phosphorylation by MARK1, MARK2 and PKA regulates its ability to bind microtubules (By similarity). Phosphorylation at Ser-265 and Ser-297 seems to occur only in neonatal brain, the levels falling precipitously by postnatal day 21 (By similarity). Ubiquitinated by MDM2, leading to its degradation by the proteasome. Ubiquitinated by MDM2 and subsequent degradation leads to reduce the dendritic spine density of olfactory bulb granule cells. |
Images (4) Click the Picture to Zoom In
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ARG52266 anti-Doublecortin antibody [3 e1] ICC/IF image
Immunofluorescence: cultured rat neurons showing strong cytoplasmic staining of doublecortin (green)(ARG52266) in developing neurons and GFAP (ARG52313) in red.
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ARG52266 anti-Doublecortin antibody [3 e1] ICC/IF image
Immunofluorescence: Cortical neuron-glial cell culture from E20 Rat stained with ARG52266 anti-Doublecortin antibody [3 e1] (red) at 1:1000 dilution, and costained with anti-MAP2 antibody (green) at 1:10000 dilution. DAPI (blue) for nuclear staining.
The Doublecortin antibody reveals strong cytoplasmic staining in a population of small developing neurons and their processes, while the MAP2 antibody stains dendrites and perikarya of mature neurons.
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ARG52266 anti-Doublecortin antibody [3 e1] WB image
Western Blot: postnatal day 3 rat brain lysate stained with Doublecortin antibody (ARG52266) showing specific immunolabeling of the ~35 & 45k doublecortin protein.
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ARG52266 anti-Doublecortin antibody [3 e1] WB image
Western blot: Adult Rat brain (negative control) and embryonic E20 Rat brain lysates stained with ARG52266 anti-Doublecortin antibody [3 e1] (green) at 1:1000 dilution.
