ARG66683
anti-Dengue virus NS4B antibody [SQab19172]
anti-Dengue virus NS4B antibody [SQab19172] for ELISA,ICC/IF,Western blot and Dengue virus
Overview
| Product Description | Mouse Monoclonal antibody [SQab19172] recognizes Dengue virus NS4B |
|---|---|
| Tested Reactivity | DEN |
| Tested Application | ELISA, ICC/IF, WB |
| Specificity | This antibody recognizes DENV2 and DENV3 Membrane protein |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SQab19172 |
| Isotype | IgG1 |
| Target Name | Dengue virus NS4B |
| Antigen Species | Dengue virus |
| Immunogen | Dengue virus NS4B. |
| Conjugation | Un-conjugated |
Application Instructions
| Application Suggestion |
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| Application Note | *Western: DENV3 not workable; ELISA: DENV3 not workable. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | PBS (pH 7.4) and 0.01% Thimerosal. |
| Preservative | 0.01% Thimerosal |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Highlight | Related products: Dengue virus antibodies; Dengue virus ELISA Kits; Dengue virus Duos / Panels; Related news: Exploring Antiviral Immune Response |
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Images (4) Click the Picture to Zoom In
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ARG66683 anti-Dengue virus NS4B antibody [SQab19172] ICC/IF image
Immunofluorescence: Mock infected Huh7 cells (upper panel) and DENV infected Huh7 cells (lower panel). Cells were fixed with 4% paraformaldehyde for 30 mins, permeabilized by 0.5% Triton-X 100 for 10 mins and blocked for 1 hour. Cells were stained with ARG66683 anti-Dengue virus NS4B antibody [SQab19172] (green) at 1:1000 dilution, overnight. Nuclear staining (blue).
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ARG66683 anti-Dengue virus NS4B antibody [SQab19172] WB image
Western blot: 10 µg of Vero, ZIKV-infected Vero and DENV2-infected Vero cells (left to right). Cell lysates were stained with ARG66683 anti-Dengue virus NS4B antibody [SQab19172] at 0.5 µg/ml dilution.
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ARG66683 anti-Dengue virus NS4B antibody [SQab19172] WB image
Western blot: 10 µg of BHK, DENV1-infected BHK, DENV2-infected BHK, DENV3-infected BHK and DENV4-infected BHK cells (left to right). Cell lysates were stained with ARG66683 anti-Dengue virus NS4B antibody [SQab19172] at 0.5 µg/ml dilution.
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ARG66683 anti-Dengue virus NS4B antibody [SQab19172] WB image
Western blot: DENV infected (right) or uninfected (left) Huh7 cells. Cell lysates were stained with ARG66683 anti-Dengue virus NS4B antibody [SQab19172] at 1:1000 dilution, overnight.
