ARG65661

anti-Dengue virus NS1 antibody [SQab1502]

Datasheet

Publication2

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100 μg

389.00

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Datasheet
Publication (2)

Overview

Product Description	Mouse Monoclonal antibody [SQab1502] recognizes Dengue virus NS1
Tested Reactivity	DEN
Tested Application	ELISA, WB
Specificity	This antibody recognizes DENV2 NS1 protein.
Host	Mouse
Clonality	Monoclonal
Clone	SQab1502
Isotype	IgG
Target Name	Dengue virus NS1
Antigen Species	Virus
Immunogen	Recombinant hexamer Dengue virus NS1 protein from drosophila cell
Conjugation	Un-conjugated
Alternate Names	Dengue virus NS1 antibody; Dengue virus nonstructural glycoprotein NS1 antibody

Application Instructions

Application Suggestion

Tested Application	Dilution
ELISA	1:1000 - 1:5000
WB	Assay-dependent

Application Note

Recommend using ARG65660 Dengue virus NS1 antibody [SQab1501] for WB, ICC/IF studies.
* The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Properties

Form	Liquid
Buffer	PBS (pH 7.4) and 0.01% Thimerosal.
Preservative	0.01% Thimerosal
Concentration	1 mg/ml
Storage Instruction	For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.
Note	For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol	DENV_gp1
Gene Full Name	Dengue virus nonstructural protein 1
Background	Dengue virus NS1 protein is a nonstructural protein which could be secreted and have been developed as diagnostic biomarker for early detection. There are several forms of NS1 including monomer, dimer, and hexamer during infection. Dimeric NS1 can be anchored to cell membranes with glycosyl-phosphatidylinositol (GPI). Hexameric NS1 can be secreted and detected in patients' blood samples (up to 50 μg/mL) or infected cell supernatants (various from ng/mL to μg/mL depend on serotypes and strains). Studies have shown that NS1 could interfere complement activity and prothrombin activation. In addition, NS1 could elicit antibodies which cross-react with host antigens including coagulation factors and molecules expressed in endothelial cells and platelets through molecular mimic.
Function	Dengue virus (DENV) non-structural protein 1 (NS1) is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome. [Uniprot]
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Research Area	Microbiology and Infectious Disease antibody

Images (1) Click the Picture to Zoom In

ARG65661 anti-Dengue virus NS1 antibody [SQab1502] & ARG65662 anti-Dengue virus NS1 antibody [SQab1503] (Biotin) ELISA image

ELISA: 293 cells expressed dengue virus type 2 NS1 protein (major in hexamer) detected by ARG65661 anti-Dengue virus NS1 antibody [SQab1502] as capture antibody (2.5 μg/ml), and ARG65662 anti-Dengue virus NS1 antibody [SQab1503] (Biotin) (0.5 μg/ml) as detection antibody, followed by incubation with streptavidin-HRP.