ARG65660
anti-Dengue virus NS1 antibody [SQab1501]
anti-Dengue virus NS1 antibody [SQab1501] for ELISA,ICC/IF,Western blot and Dengue virus
Microbiology and Infectious Disease antibody
Publication6
Overview
| Product Description | Mouse Monoclonal antibody [SQab1501] recognizes Dengue virus NS1 |
|---|---|
| Tested Reactivity | DEN |
| Tested Application | ELISA, ICC/IF, WB |
| Specificity | This antibody recognizes monomer, dimer, hexamer of DENV1/2/3/4 NS1 protein. |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | SQab1501 |
| Isotype | IgG2a |
| Target Name | Dengue virus NS1 |
| Antigen Species | Virus |
| Immunogen | Recombinant hexamer Dengue virus NS1 protein from drosophila cell |
| Conjugation | Un-conjugated |
| Alternate Names | Dengue virus NS1 antibody; Dengue virus nonstructural glycoprotein NS1 antibody |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Buffer | PBS (pH 7.4), 0.01% Thimerosal, 1% BSA and 10% Glycerol. |
| Preservative | 0.01% Thimerosal |
| Stabilizer | 1% BSA and 10% Glycerol |
| Concentration | 1 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | DENV_gp1 |
|---|---|
| Gene Full Name | Dengue virus nonstructural protein 1 |
| Background | Dengue virus NS1 protein is a nonstructural protein which could be secreted and have been developed as diagnostic biomarker for early detection. There are several forms of NS1 including monomer, dimer, and hexamer during infection. Dimeric NS1 can be anchored to cell membranes with glycosyl-phosphatidylinositol (GPI). Hexameric NS1 can be secreted and detected in patients' blood samples (up to 50 μg/mL) or infected cell supernatants (various from ng/mL to μg/mL depend on serotypes and strains). Studies have shown that NS1 could interfere complement activity and prothrombin activation. In addition, NS1 could elicit antibodies which cross-react with host antigens including coagulation factors and molecules expressed in endothelial cells and platelets through molecular mimic. |
| Function | Dengue virus (DENV) non-structural protein 1 (NS1) is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome. [Uniprot] |
| Highlight | Related products: Dengue Virus antibodies; Dengue Virus ELISA Kits; Dengue Virus Duos / Panels; Anti-Mouse IgG secondary antibodies; Related news: Best NS1 antibodies in the market Fighting fire with fire: Genetically-engineered mosquitoes as an alternative to fight diseases Tools for studying Dengue Virus Exploring Antiviral Immune Response |
| Research Area | Microbiology and Infectious Disease antibody |
Images (3) Click the Picture to Zoom In
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ARG65660 anti-Dengue virus NS1 antibody [SQab1501] ICC/IF image
Immunofluorescence: ARG65660 anti-Dengue virus NS1 antibody [SQab1501] (1:400) were used for detecting DENV NS1.
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ARG65660 anti-Dengue virus NS1 antibody [SQab1501] WB image
Western blot: 6 μg of Vero cells Infected with 1) Mock, 2) ZIKV, 3) DENV1, 4) DENV2, 5) DENV3 and 6) DENV 4. Cell lysates were stained with ARG65660 anti-Dengue virus NS1 antibody [SQab1501] at 1:2000.
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ARG65660 anti-Dengue virus NS1 antibody [SQab1501] WB image
Western blot: 6 μg of DENV2-infected C636 and ZIKV-infected C636 cell lysates stained with ARG65660 anti-Dengue virus NS1 antibody [SQab1501] at 0.5 μg/ml dilution.
Customer's Feedback
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Review for anti-Dengue virus NS1 antibody [SQab1501]
Application：FACS
Sample：Dengue virus type 2 (strain 16681) infected monocyte cells (M.O.I.=10)
Cell Number/Concentration：1x10E6
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：1hr
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：AlexaFluor488
Specific References
SARS-CoV-2 N protein enhances the anti-apoptotic activity of MCL-1 to promote viral replication
WB / Dengue virus
NS1 Protein N-Linked Glycosylation Site Affects the Virulence and Pathogenesis of Dengue Virus
WB / Dengue Virus
N-Butanol Extract of Glycyrrhizae Radix et Rhizoma Inhibits Dengue Virus through Targeting Envelope Protein
WB, ICC/IF / Dengue virus
A Novel Role for the Regulatory Nod-Like Receptor NLRP12 in Anti-Dengue Virus Response
WB / Dengue virus
DENV NS1 and MMP-9 cooperate to induce vascular leakage by altering endothelial cell adhesion and tight junction.
WB, ICC/IF / Dengue virus
MicroRNA-155 inhibits dengue virus replication by inducing heme oxygenase-1-mediated antiviral interferon responses.
IHC-P / Dengue virus
