ARG67001
anti-Dengue virus NS1 antibody
anti-Dengue virus NS1 antibody for ICC/IF,Western blot and Dengue virus
Microbiology and Infectious Disease antibody
Overview
| Product Description | Rabbit Polyclonal antibody recognizes Dengue virus NS1 |
|---|---|
| Tested Reactivity | DEN |
| Tested Application | ICC/IF, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | Dengue virus NS1 |
| Antigen Species | Virus |
| Immunogen | Recombinant protein corresponding to Dengue virus NS1 protein. |
| Conjugation | Un-conjugated |
| Alternate Names | Dengue virus NS1 antibody; Dengue virus nonstructural glycoprotein NS1 antibody |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified. |
| Buffer | 100 mM Tris Glycine (pH 7.0), 0.025% ProClin 300 and 20% Glycerol. |
| Preservative | 0.025% ProClin 300 |
| Stabilizer | 20% Glycerol |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Gene Symbol | DENV_gp1 |
|---|---|
| Gene Full Name | Dengue virus nonstructural protein 1 |
| Background | Dengue virus NS1 protein is a nonstructural protein which could be secreted and have been developed as diagnostic biomarker for early detection. There are several forms of NS1 including monomer, dimer, and hexamer during infection. Dimeric NS1 can be anchored to cell membranes with glycosyl-phosphatidylinositol (GPI). Hexameric NS1 can be secreted and detected in patients' blood samples (up to 50 μg/mL) or infected cell supernatants (various from ng/mL to μg/mL depend on serotypes and strains). Studies have shown that NS1 could interfere complement activity and prothrombin activation. In addition, NS1 could elicit antibodies which cross-react with host antigens including coagulation factors and molecules expressed in endothelial cells and platelets through molecular mimic. |
| Function | Dengue virus (DENV) non-structural protein 1 (NS1) is involved in virus replication and regulation of the innate immune response. Soluble and membrane-associated NS1 may activate human complement and induce host vascular leakage. This effect might explain the clinical manifestations of dengue hemorrhagic fever and dengue shock syndrome. [Uniprot] |
| Highlight | Related products: Dengue Virus antibodies; Dengue Virus ELISA Kits; Dengue Virus Duos / Panels; Anti-Rabbit IgG secondary antibodies; Related news: Best NS1 antibodies in the market Fighting fire with fire: Genetically-engineered mosquitoes as an alternative to fight diseases Tools for studying Dengue Virus Exploring Antiviral Immune Response |
| Research Area | Microbiology and Infectious Disease antibody |
Images (2) Click the Picture to Zoom In
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ARG67001 anti-Dengue virus NS1 antibody ICC/IF image
Immunofluorescence: DENV2 infected Vero cells or mock infected Vero cells were fixed with 4% paraformaldehyde at RT for 10 min, permeabilized with 0.1% NP-40 at RT for 10 min and then cells were blocked with 5% BSA at RT for 30 min and stained with ARG67001 anti-Dengue virus NS1 antibody .
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ARG67001 anti-Dengue virus NS1 antibody WB image
Western blot: Lysate from BHK-21 cell infected with DENV2 stained with ARG67001 anti-Dengue virus NS1 antibody at 1:1000 dilution, overnight at 4°C.
