ARG62468
anti-DNA polymerase beta antibody [18S]
anti-DNA polymerase beta antibody [18S] for IHC-Formalin-fixed paraffin-embedded sections,Immunohistochemistry,Immunoprecipitation,Western blot and Bovine,Hamster,Human,Mouse,Rat,Xenopus laevis
Gene Regulation antibody
Overview
| Product Description | Mouse Monoclonal antibody [18S] recognizes DNA polymerase beta |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Bov, Hm, Xenopus laevis |
| Tested Application | IHC, IHC-P, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Clone | 18S |
| Isotype | IgG1 |
| Target Name | DNA polymerase beta |
| Antigen Species | Rat |
| Immunogen | Rat DNA polymerase beta full length protein (Rat) |
| Conjugation | Un-conjugated |
| Alternate Names | EC 4.2.99.-; EC 2.7.7.7; DNA polymerase beta |
Application Instructions
| Application Note | WB: 1-2 ug/ml IHC: 1/10-1/500 IP: 2 ug/ml * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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Properties
| Form | Liquid |
|---|---|
| Buffer | 1X PBS buffer with sodium azide (less than 0.1%). |
| Preservative | sodium azide (less than 0.1%) |
| Concentration | 2 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | Polb |
| Gene Full Name | polymerase (DNA directed), beta |
| Background | The protein encoded by this gene is a DNA polymerase involved in base excision and repair, also called gap-filling DNA synthesis. The encoded protein, acting as a monomer, is normally found in the cytoplasm, but it translocates to the nucleus upon DNA damage. Several transcript variants of this gene exist, but the full-length nature of only one has been described to date. [provided by RefSeq, Sep 2011] |
| Function | Repair polymerase that plays a key role in base-excision repair. Has 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity that removes the 5' sugar phosphate and also acts as a DNA polymerase that adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. [UniProt] |
| Cellular Localization | Nucleus |
| Research Area | Gene Regulation antibody |
| Calculated MW | 38 kDa |
| PTM | Methylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity. Ubiquitinated at Lys-41, Lys-61 and Lys-81: monoubiquitinated by HUWE1/ARF-BP1. Monoubiquitinated protein is then the target of STUB1/CHIP, which catalyzes polyubiquitination from monoubiquitin, leading to degradation by the proteasome. USP47 mediates the deubiquitination of monoubiquitinated protein, preventing polyubiquitination by STUB1/CHIP and its subsequent degradation. |
Clone References
Base excision repair defects invoke hypersensitivity to PARP inhibition.
IP / Mouse
Differential regulation of S-region hypermutation and class-switch recombination by noncanonical functions of uracil DNA glycosylase.
An inverse switch in DNA base excision and strand break repair contributes to melphalan resistance in multiple myeloma cells.
WB / Human
Oxidative stress alters base excision repair pathway and increases apoptotic response in apurinic/apyrimidinic endonuclease 1/redox factor-1 haploinsufficient mice.
WB / Mouse
DNA polymerase beta is able to repair breaks in switch regions and plays an inhibitory role during immunoglobulin class switch recombination.
WB, ChIP / Mouse
A new XRCC1-containing complex and its role in cellular survival of methyl methanesulfonate treatment.
ChIP / Human
Oxidative DNA damage and repair in experimental atherosclerosis are reversed by dietary lipid lowering.
WB / Rabbit
