ARG64688
anti-DMP1 antibody
anti-DMP1 antibody for ICC/IF,Western blot and Human
Developmental Biology antibody; Signaling Transduction antibody
Publication3
Overview
| Product Description | Goat Polyclonal antibody recognizes DMP1 |
|---|---|
| Tested Reactivity | Hu |
| Tested Application | ICC/IF, WB |
| Specificity | This antibody is expected to recognize both reported isoforms (NP_004398.1; NP_001073380.1). |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DMP1 |
| Antigen Species | Human |
| Immunogen | C-ENSEQANTQDSG |
| Conjugation | Un-conjugated |
| Alternate Names | DMP-1; Dentin matrix protein 1; ARHP; Dentin matrix acidic phosphoprotein 1; ARHR |
Application Instructions
| Application Suggestion |
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| Application Note | WB: Recommend incubate at RT for 1h. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # Q13316 Human Dentin matrix acidic phosphoprotein 1 |
|---|---|
| Background | Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
| Research Area | Developmental Biology antibody; Signaling Transduction antibody |
| Calculated MW | 56 kDa |
| PTM | Phosphorylated in the cytosol and extracellular matrix and unphosphorylated in the nucleus. Phosphorylation is necessary for nucleocytoplasmic transport and may be catalyzed by a nuclear isoform of CK2 and can be augmented by calcium. Phosphorylated (in vitro) by FAM20C in the extracellular medium at sites within the S-x-E/pS motif. |
Images (3) Click the Picture to Zoom In
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ARG64688 anti-NGEF Antibody WB image
Western blot: human dental pulp stem cells (hDPSCs) stained with ARG64688 anti-NGEF Antibody.
From Kim Hong-Bae et al. Sci Rep. (2018), doi: 10.1038/s41598-018-19395-x, Fig. 5C.
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ARG64688 anti-DMP1 antibody ICC/IF image
Immunofluorescence: WJMSC cells fixed with 10% Formalin for 20 min and blocked with PBS, 5% FBS and 0.1% Triton at RT for 30 min. Cells were incubated in PBS with 5% FBS and stained with ARG64688 anti-DMP1 antibody (green) at 1:200 dilution, overnight at 4°C. DAPI (blue) for nuclear staining.
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ARG64688 anti-DMP1 antibody WB image
Western blot: 35 µg of Human kidney lysate (in RIPA buffer) stained with ARG64688 anti-DMP1 antibody at 1 µg/ml dilution and incubated at RT for 1 hour.
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Review for anti-DMP1 antibody
Application：IF/ICC
Sample：WJMSC
Fixation Buffer：10% Formalin
Fixation Time：20 min
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：Alexa 488
Specific References
Development of hybrid scaffolds with biodegradable polymer composites and bioactive hydrogels for bone tissue engineering
ICC/IF / Human
Pulse Frequency Dependency of Photobiomodulation on Osteogenic Differentiation of Human Dental Pulp Stem Cells.
WB / Human
Effects of pulsing of light on the dentinogenesis of dental pulp stem cells in vitro.
WB / Human
