ARG63435
anti-DDX5 / p68 RNA helicase antibody
anti-DDX5 / p68 RNA helicase antibody for ICC/IF,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse
Gene Regulation antibody
Overview
| Product Description | Goat Polyclonal antibody recognizes DDX5 / p68 RNA helicase |
|---|---|
| Tested Reactivity | Hu, Ms |
| Predict Reactivity | Dog, Rat |
| Tested Application | ICC/IF, IHC-P, WB |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DDX5 / p68 RNA helicase |
| Antigen Species | Human |
| Immunogen | C-PMIGYPMPTGYSQ |
| Conjugation | Un-conjugated |
| Alternate Names | Probable ATP-dependent RNA helicase DDX5; DEAD box protein 5; p68; HUMP68; EC 3.6.4.13; G17P1; RNA helicase p68; HLR1 |
Application Instructions
| Application Suggestion |
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|---|---|---|---|---|---|---|---|---|---|
| Application Note | WB: Recommend incubate at RT for 1h. IHC-P: Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | Tris saline (pH 7.3), 0.02% Sodium azide and 0.5% BSA |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links |
Swiss-port # P17844 Human Probable ATP-dependent RNA helicase DDX5 |
|---|---|
| Background | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a RNA-dependent ATPase, and also a proliferation-associated nuclear antigen, specifically reacting with the simian virus 40 tumor antigen. This gene consists of 13 exons, and alternatively spliced transcripts containing several intron sequences have been detected, but no isoforms encoded by these transcripts have been identified. [provided by RefSeq, Jul 2008] |
| Research Area | Gene Regulation antibody |
| Calculated MW | 69 kDa |
| PTM | Arg-502 is dimethylated, probably to asymmetric dimethylarginine. Sumoylated; sumoylation, promoted by PIAS1, promotes interaction with HDAC1 and transcriptional repression activity. Sumoylation also significantly increases stability, and reduces polyubiquitination. Polyubiquitinated, leading to proteasomal degradation. |
Images (4) Click the Picture to Zoom In
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ARG63435 anti-DDX5 / p68 RNA helicase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human uterus tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63435 anti-DDX5 / p68 RNA helicase antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG63435 anti-DDX5 / p68 RNA helicase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human prostate tissue. (Steamed antigen retrieval with citrate buffer pH 6) stained with ARG63435 anti-DDX5 / p68 RNA helicase antibody at 2.5 µg/ml dilution followed by AP-staining.
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ARG63435 anti-DDX5 / p68 RNA helicase antibody WB image
Western blot: 35 µg of A431 (A), HeLa (B), HepG2 (C), Jurkat (D) and NIH/3T3 (E) cell lysates (in RIPA buffer) stained with ARG63435 anti-DDX5 / p68 RNA helicase antibody at 0.5 µg/ml dilution and incubated at RT for 1 hour.
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ARG63435 anti-DDX5 / p68 RNA helicase antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human breast tissue. Antigen Retrieval: Steam tissue section in Citrate buffer (pH 6.0). The tissue section was stained with ARG63435 anti-DDX5 / p68 RNA helicase antibody at 2.5 µg/ml dilution followed by AP-staining.
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Review for anti-DDX5 / p68 RNA helicase antibody
Application：WB
Sample：293T and HeLa cells
Sample Loading Amount：20 µg
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
