ARG41563
anti-DDX4 antibody
anti-DDX4 antibody for Flow cytometry,ICC/IF,IHC-Frozen sections,IHC-Formalin-fixed paraffin-embedded sections,Western blot and Human,Mouse,Rat
Overview
| Product Description | Rabbit Polyclonal antibody recognizes DDX4 |
|---|---|
| Tested Reactivity | Hu, Ms, Rat |
| Predict Reactivity | Hm |
| Tested Application | FACS, ICC/IF, IHC-Fr, IHC-P, WB |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Target Name | DDX4 |
| Antigen Species | Human |
| Immunogen | Synthetic peptide corresponding to aa. 253-272 of Human DDX4. (EDEDSIFAHYQTGINFDKYD) |
| Conjugation | Un-conjugated |
| Alternate Names | Probable ATP-dependent RNA helicase DDX4; VASA; Vasa homolog; DEAD box protein 4; EC 3.6.4.13 |
Application Instructions
| Application Suggestion |
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| Application Note | IHC-P: Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
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| Observed Size | ~ 80 kDa |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purification with immunogen. |
| Buffer | 0.2% Na2HPO4, 0.9% NaCl, 0.05% Thimerosal, 0.05% Sodium azide and 5% BSA. |
| Preservative | 0.05% Thimerosal and 0.05% Sodium azide |
| Stabilizer | 5% BSA |
| Concentration | 0.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
|---|---|
| Gene Symbol | DDX4 |
| Gene Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 4 |
| Background | DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a homolog of VASA proteins in Drosophila and several other species. The gene is specifically expressed in the germ cell lineage in both sexes and functions in germ cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009] |
| Function | May play a role in germ cell development. May play a role in sperm motility. [UniProt] |
| Cellular Localization | Cytoplasm. Cytoplasm, perinuclear region. Note=Component of the meiotic nuage, also named P granule, a germ-cell-specific organelle required to repress transposon activity during meiosis. [UniProt] |
| Calculated MW | 79 kDa |
Images (9) Click the Picture to Zoom In
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ARG41563 anti-DDX4 antibody ICC/IF image
Immunocytochemistry: MCF7 cells stained with ARG41563 anti-DDX4 antibody.
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ARG41563 anti-DDX4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat ovary tissue stained with ARG41563 anti-DDX4 antibody.
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ARG41563 anti-DDX4 antibody WB image
Western blot: Rat testis, Mouse testis and HeLa cell lysates stained with ARG41563 anti-DDX4 antibody.
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ARG41563 anti-DDX4 antibody FACS image
Flow Cytometry: SiHa cells were blocked with 10% normal Goat serum and then stained with ARG41563 anti-DDX4 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
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ARG41563 anti-DDX4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Rat testis tissue stained with ARG41563 anti-DDX4 antibody.
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ARG41563 anti-DDX4 antibody IHC-Fr image
Immunohistochemistry: Frozen section of Rat intestine tissue stained with ARG41563 anti-DDX4 antibody.
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ARG41563 anti-DDX4 antibody IHC-P image
Immunohistochemistry: Paraffin-embedded Human ovarian cancer tissue. Antigen Retrieval: Heat mediation was performed in Citrate buffer (pH 6.0, epitope retrieval solution) for 20 min. The tissue section was blocked with 10% goat serum. The tissue section was then stained with ARG41563 anti-DDX4 antibody at 1 µg/ml dilution, overnight at 4°C.
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ARG41563 anti-DDX4 antibody WB image
Western blot: Rat testis, Mouse testis and Mouse ovary lysates stained with ARG41563 anti-DDX4 antibody.
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ARG41563 anti-DDX4 antibody FACS image
Flow Cytometry: PC-3 cells were blocked with 10% normal Goat serum and then stained with ARG41563 anti-DDX4 antibody (blue) at 1 µg/10^6 cells for 30 min at 20°C, followed by incubation with DyLight®488 labelled secondary antibody. Isotype control antibody (green) was Rabbit IgG (1 µg/10^6 cells) used under the same conditions. Unlabelled sample (red) was also used as a control.
