ARG54169
anti-DDX3 antibody
anti-DDX3 antibody for ICC/IF,Immunoprecipitation,Western blot and Human,Mouse,Rat,Monkey
Developmental Biology antibody; Gene Regulation antibody
Publication1
Overview
| Product Description | Mouse Monoclonal antibody recognizes DDX3X |
|---|---|
| Tested Reactivity | Hu, Ms, Rat, Mk |
| Tested Application | ICC/IF, IP, WB |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Target Name | DDX3 |
| Antigen Species | Human |
| Immunogen | Purified recombinant human DDX3 protein fragments expressed in E.coli. |
| Conjugation | Un-conjugated |
| Alternate Names | DDX14; DEAD box protein 3, X-chromosomal; CAP-Rf; Helicase-like protein 2; DEAD box, X isoform; DBX; HLP2; EC 3.6.4.13; DDX3; ATP-dependent RNA helicase DDX3X |
Application Instructions
| Application Suggestion |
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| Application Note | * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. |
Properties
| Form | Liquid |
|---|---|
| Purification | Affinity purified |
| Buffer | PBS (pH 7.4), 0.02% Sodium azide, 0.1mg/ml BSA and 50% Glycerol |
| Preservative | 0.02% Sodium azide |
| Stabilizer | 0.1mg/ml BSA, 50% Glycerol |
| Concentration | 1.5 mg/ml |
| Storage Instruction | For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. |
| Note | For laboratory research only, not for drug, diagnostic or other use. |
Bioinformation
| Database Links | |
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| Gene Symbol | DDX3X |
| Gene Full Name | DEAD (Asp-Glu-Ala-Asp) box helicase 3, X-linked |
| Background | The protein encoded by this gene is a member of the large DEAD-box protein family, that is defined by the presence of the conserved Asp-Glu-Ala-Asp (DEAD) motif, and has ATP-dependent RNA helicase activity. This protein has been reported to display a high level of RNA-independent ATPase activity, and unlike most DEAD-box helicases, the ATPase activity is thought to be stimulated by both RNA and DNA. This protein has multiple conserved domains and is thought to play roles in both the nucleus and cytoplasm. Nuclear roles include transcriptional regulation, mRNP assembly, pre-mRNA splicing, and mRNA export. In the cytoplasm, this protein is thought to be involved in translation, cellular signaling, and viral replication. Misregulation of this gene has been implicated in tumorigenesis. This gene has a paralog located in the nonrecombining region of the Y chromosome. Pseudogenes sharing similarity to both this gene and the DDX3Y paralog are found on chromosome 4 and the X chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
| Function | Multifunctional ATP-dependent RNA helicase. The ATPase activity can be stimulated by various ribo- and deoxynucleic acids indicative for a relaxed substrate specificity. In vitro can unwind partially double-stranded DNA with a preference for 5'-single-stranded DNA overhangs. Is involved in several steps of gene expression, such as transcription, mRNA maturation, mRNA export and translation. However, the exact mechanisms are not known and some functions may be specific for a subset of mRNAs. Involved in transcriptional regulation. Can enhance transcription from the CDKN1A/WAF1 promoter in a SP1-dependent manner. Found associated with the E-cadherin promoter and can down-regulate transcription from the promoter. Involved in regulation of translation initiation. Proposed to be involved in positive regulation of translation such as of cyclin E1/CCNE1 mRNA and specifically of mRNAs containing complex secondary structures in their 5'UTRs; these functions seem to require RNA helicase activity. Specifically promotes translation of a subset of viral and cellular mRNAs carrying a 5'proximal stem-loop structure in their 5'UTRs and cooperates with the eIF4F complex. Proposed to act prior to 43S ribosomal scanning and to locally destabilize these RNA structures to allow recognition of the mRNA cap or loading onto the 40S subunit. After association with 40S ribosomal subunits seems to be involved in the functional assembly of 80S ribosomes; the function seems to cover translation of mRNAs with structured and non-structured 5'UTRs and is independent of RNA helicase activity. [UniProt] |
| Cellular Localization | Nucleus speckle. Cytoplasm. Mitochondrion outer membrane. Note: Located predominantly in nuclear speckles and, at low levels, throughout the cytoplasm. Located to the outer side of nuclear pore complexes (NPC). Shuttles between the nucleus and the cytoplasm in a XPO1 and may be also in a NFX1-dependent manner. Associated with polyadenylated mRNAs in the cytoplasm and the nucleus. Predominantly located in nucleus during G0 phase and in the cytoplasm during G1/S phase. |
| Research Area | Developmental Biology antibody; Gene Regulation antibody |
| Calculated MW | 73 kDa |
| PTM | Phosphorylated by TBK1; the phosphorylation is required to synergize with TBK1 in IFNB induction. Phosphorylated by IKBKE at Ser-102 after ssRNA viral infection; enhances the induction of INFB promoter by IRF3. The cytoplasmic form is highly phosphorylated in the G1/S phase and much lower phosphorylated in G2/M. |
Images (4) Click the Picture to Zoom In
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ARG54169 anti-DDX3 antibody ICC/IF image
Immunofluorescence: 100% Methanol fixed (RT, 10 min) HeLa cells stained with ARG54169 anti-DDX3 antibody at 1:200 dilution. Left: primary antibody (green). Right: DAPI (blue).
Secondary antibody: ARG55393 Goat anti-Mouse IgG (H+L) antibody (FITC)
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ARG54169 anti-DDX3 antibody WB image
Western blot: 30 µg of PC3 and DU145 cell lysates stained with ARG54169 anti-DDX3 antibody at 1:1000 dilution.
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ARG54169 anti-DDX3 antibody WB image
Western blot: 30 µg of Mouse spleen lysate stained with ARG54169 anti-DDX3 antibody at 1:1000 dilution.
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ARG54169 anti-DDX3 antibody IP image
Immunoprecipitation: HeLa cell lysates were immunoprecipitated and stained with ARG54169 anti-DDX3 antibody.
Customer's Feedback
Excellent
Review for anti-DDX3 antibody
Application：IF/ICC
Sample：HeLa
Fixation Buffer：100% Methanol
Fixation Time：10 min
Fixation Temperature：RT ºC
Permeabilization Buffer：0.1% Triton X-100
Primary Antibody Dilution Factor：1:200
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Conjugation of Secondary Antibody：FITC
Excellent
Review for anti-DDX3 antibody
Application：WB
Sample：PC3 and DU145
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-DDX3 antibody
Application：WB
Sample：Mouse spleen
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Good
Review for anti-DDX3 antibody
Application：WB
Sample：Jurkat
Sample Loading Amount：30 µg
Primary Antibody Dilution Factor：1:1000
Primary Antibody Incubation Time：overnight
Primary Antibody Incubation Temperature：4 ºC
Specific References
Arginine Methylation of hnRNPK Inhibits the DDX3-hnRNPK Interaction to Play an Anti-Apoptosis Role in Osteosarcoma Cells
WB / Human
